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Meso Scale Discovery and Luminex Comparative Analysis of Calbindin D28K

The sensitivity of different renal regions to xenobiotics requires the development of a multiplex immunoassay for the simultaneous analysis of kidney biomarkers. Calbindin D28K is a distal tubule-specific protein that can be detected in urine under pathological conditions. In this study, a pair of a...

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Detalles Bibliográficos
Autores principales: Sourial, Samer, Marcusson-Ståhl, Maritha, Cederbrant, Karin
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2760132/
https://www.ncbi.nlm.nih.gov/pubmed/19826628
http://dx.doi.org/10.1155/2009/187426
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author Sourial, Samer
Marcusson-Ståhl, Maritha
Cederbrant, Karin
author_facet Sourial, Samer
Marcusson-Ståhl, Maritha
Cederbrant, Karin
author_sort Sourial, Samer
collection PubMed
description The sensitivity of different renal regions to xenobiotics requires the development of a multiplex immunoassay for the simultaneous analysis of kidney biomarkers. Calbindin D28K is a distal tubule-specific protein that can be detected in urine under pathological conditions. In this study, a pair of anti-calbindin D28K antibodies was used in an immunoassay for the detection of calbindin D28K expression in rat and human kidney and urine. Comparative analysis of the immunoassay was performed on the Meso Scale Development (MSD) and Luminex platforms. Analysis on both platforms detected calbindin D28K concentrations between 100 ng/mL and 100 pg/mL. Luminex detected 10-fold the amount of calbindin D28K in samples analyzed as compared to MSD, whereas calbindin D28K level in rat and human urine was below detection limit in both platforms. The application of the immunoassays described herein may be useful in toxicological and pathological studies of distal tubular damage in rats and human.
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spelling pubmed-27601322009-10-13 Meso Scale Discovery and Luminex Comparative Analysis of Calbindin D28K Sourial, Samer Marcusson-Ståhl, Maritha Cederbrant, Karin J Biomed Biotechnol Research Article The sensitivity of different renal regions to xenobiotics requires the development of a multiplex immunoassay for the simultaneous analysis of kidney biomarkers. Calbindin D28K is a distal tubule-specific protein that can be detected in urine under pathological conditions. In this study, a pair of anti-calbindin D28K antibodies was used in an immunoassay for the detection of calbindin D28K expression in rat and human kidney and urine. Comparative analysis of the immunoassay was performed on the Meso Scale Development (MSD) and Luminex platforms. Analysis on both platforms detected calbindin D28K concentrations between 100 ng/mL and 100 pg/mL. Luminex detected 10-fold the amount of calbindin D28K in samples analyzed as compared to MSD, whereas calbindin D28K level in rat and human urine was below detection limit in both platforms. The application of the immunoassays described herein may be useful in toxicological and pathological studies of distal tubular damage in rats and human. Hindawi Publishing Corporation 2009 2009-10-11 /pmc/articles/PMC2760132/ /pubmed/19826628 http://dx.doi.org/10.1155/2009/187426 Text en Copyright © 2009 Samer Sourial et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sourial, Samer
Marcusson-Ståhl, Maritha
Cederbrant, Karin
Meso Scale Discovery and Luminex Comparative Analysis of Calbindin D28K
title Meso Scale Discovery and Luminex Comparative Analysis of Calbindin D28K
title_full Meso Scale Discovery and Luminex Comparative Analysis of Calbindin D28K
title_fullStr Meso Scale Discovery and Luminex Comparative Analysis of Calbindin D28K
title_full_unstemmed Meso Scale Discovery and Luminex Comparative Analysis of Calbindin D28K
title_short Meso Scale Discovery and Luminex Comparative Analysis of Calbindin D28K
title_sort meso scale discovery and luminex comparative analysis of calbindin d28k
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2760132/
https://www.ncbi.nlm.nih.gov/pubmed/19826628
http://dx.doi.org/10.1155/2009/187426
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