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Review of Current Methodological Approaches for Characterizing MicroRNAs in Plants

Advances in molecular biology have led to some surprising discoveries. One of these includes the complexities of RNA and its role in gene expression. One particular class of RNA called microRNA (miRNA) is the focus of this paper. We will first briefly look at some of the characteristics and biogenes...

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Detalles Bibliográficos
Autores principales: Unver, Turgay, Namuth-Covert, Deana M., Budak, Hikmet
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2760397/
https://www.ncbi.nlm.nih.gov/pubmed/19834623
http://dx.doi.org/10.1155/2009/262463
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author Unver, Turgay
Namuth-Covert, Deana M.
Budak, Hikmet
author_facet Unver, Turgay
Namuth-Covert, Deana M.
Budak, Hikmet
author_sort Unver, Turgay
collection PubMed
description Advances in molecular biology have led to some surprising discoveries. One of these includes the complexities of RNA and its role in gene expression. One particular class of RNA called microRNA (miRNA) is the focus of this paper. We will first briefly look at some of the characteristics and biogenesis of miRNA in plant systems. The remainder of the paper will go into details of three different approaches used to identify and study miRNA. These include two reverse genetics approaches: computation (bioinformatics) and experimental, and one rare forward genetics approach. We also will summarize how to measure and quantify miRNAs, and how to detect their possible targets in plants. Strengths and weaknesses of each methodological approach are discussed.
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spelling pubmed-27603972009-10-15 Review of Current Methodological Approaches for Characterizing MicroRNAs in Plants Unver, Turgay Namuth-Covert, Deana M. Budak, Hikmet Int J Plant Genomics Review Article Advances in molecular biology have led to some surprising discoveries. One of these includes the complexities of RNA and its role in gene expression. One particular class of RNA called microRNA (miRNA) is the focus of this paper. We will first briefly look at some of the characteristics and biogenesis of miRNA in plant systems. The remainder of the paper will go into details of three different approaches used to identify and study miRNA. These include two reverse genetics approaches: computation (bioinformatics) and experimental, and one rare forward genetics approach. We also will summarize how to measure and quantify miRNAs, and how to detect their possible targets in plants. Strengths and weaknesses of each methodological approach are discussed. Hindawi Publishing Corporation 2009 2009-10-08 /pmc/articles/PMC2760397/ /pubmed/19834623 http://dx.doi.org/10.1155/2009/262463 Text en Copyright © 2009 Turgay Unver et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Unver, Turgay
Namuth-Covert, Deana M.
Budak, Hikmet
Review of Current Methodological Approaches for Characterizing MicroRNAs in Plants
title Review of Current Methodological Approaches for Characterizing MicroRNAs in Plants
title_full Review of Current Methodological Approaches for Characterizing MicroRNAs in Plants
title_fullStr Review of Current Methodological Approaches for Characterizing MicroRNAs in Plants
title_full_unstemmed Review of Current Methodological Approaches for Characterizing MicroRNAs in Plants
title_short Review of Current Methodological Approaches for Characterizing MicroRNAs in Plants
title_sort review of current methodological approaches for characterizing micrornas in plants
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2760397/
https://www.ncbi.nlm.nih.gov/pubmed/19834623
http://dx.doi.org/10.1155/2009/262463
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