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Characterization of the Gene BmEm4, a Homologue of Drosophila E(spl)m4, from the Silkworm, Bombyx mori

The Drosophila E(spl)m4 gene contains some highly conserved motifs (such as the Brd box, GY box, K box, and CAAC motif) in its 3′ untranslated region (3′ UTR). It was shown to be a microRNA target gene in Drosophila and to play an important role in the regulation of neurogenesis. We identified a hom...

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Autores principales: Zeng, Fenghui, Xie, Hongxia, Nie, Zuoming, Chen, Jian, Lv, Zhengbing, Chen, Jianqing, Wang, Dan, Liu, Lili, Yu, Wei, Sheng, Qing, Wu, Xiangfu, Zhang, Yaozhou
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2760746/
https://www.ncbi.nlm.nih.gov/pubmed/19830255
http://dx.doi.org/10.1155/2009/947490
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author Zeng, Fenghui
Xie, Hongxia
Nie, Zuoming
Chen, Jian
Lv, Zhengbing
Chen, Jianqing
Wang, Dan
Liu, Lili
Yu, Wei
Sheng, Qing
Wu, Xiangfu
Zhang, Yaozhou
author_facet Zeng, Fenghui
Xie, Hongxia
Nie, Zuoming
Chen, Jian
Lv, Zhengbing
Chen, Jianqing
Wang, Dan
Liu, Lili
Yu, Wei
Sheng, Qing
Wu, Xiangfu
Zhang, Yaozhou
author_sort Zeng, Fenghui
collection PubMed
description The Drosophila E(spl)m4 gene contains some highly conserved motifs (such as the Brd box, GY box, K box, and CAAC motif) in its 3′ untranslated region (3′ UTR). It was shown to be a microRNA target gene in Drosophila and to play an important role in the regulation of neurogenesis. We identified a homologue of the E(spl)m4 gene from Bombyx mori called BmEm4 and examined the expression patterns of BmEm4 mRNA and protein. There was a lack of correlation in the expression of the mRNA and protein between the different developmental stages, which raises the possibility of posttranscriptional regulation of the BmEm4 mRNA. Consistent with this idea is the finding that the 3′ UTR contains two putative binding sites for microRNAs. Moreover, given that the expression is the highest in the larval head, as confirmed by immunohistochemistry, we propose that BmEm4 may also be involved in the regulation of neurogenesis. Immunostaining indicated that BmEm4 is located primarily in the cytoplasm.
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spelling pubmed-27607462009-10-14 Characterization of the Gene BmEm4, a Homologue of Drosophila E(spl)m4, from the Silkworm, Bombyx mori Zeng, Fenghui Xie, Hongxia Nie, Zuoming Chen, Jian Lv, Zhengbing Chen, Jianqing Wang, Dan Liu, Lili Yu, Wei Sheng, Qing Wu, Xiangfu Zhang, Yaozhou Comp Funct Genomics Research Article The Drosophila E(spl)m4 gene contains some highly conserved motifs (such as the Brd box, GY box, K box, and CAAC motif) in its 3′ untranslated region (3′ UTR). It was shown to be a microRNA target gene in Drosophila and to play an important role in the regulation of neurogenesis. We identified a homologue of the E(spl)m4 gene from Bombyx mori called BmEm4 and examined the expression patterns of BmEm4 mRNA and protein. There was a lack of correlation in the expression of the mRNA and protein between the different developmental stages, which raises the possibility of posttranscriptional regulation of the BmEm4 mRNA. Consistent with this idea is the finding that the 3′ UTR contains two putative binding sites for microRNAs. Moreover, given that the expression is the highest in the larval head, as confirmed by immunohistochemistry, we propose that BmEm4 may also be involved in the regulation of neurogenesis. Immunostaining indicated that BmEm4 is located primarily in the cytoplasm. Hindawi Publishing Corporation 2009 2009-10-12 /pmc/articles/PMC2760746/ /pubmed/19830255 http://dx.doi.org/10.1155/2009/947490 Text en Copyright © 2009 Fenghui Zeng et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zeng, Fenghui
Xie, Hongxia
Nie, Zuoming
Chen, Jian
Lv, Zhengbing
Chen, Jianqing
Wang, Dan
Liu, Lili
Yu, Wei
Sheng, Qing
Wu, Xiangfu
Zhang, Yaozhou
Characterization of the Gene BmEm4, a Homologue of Drosophila E(spl)m4, from the Silkworm, Bombyx mori
title Characterization of the Gene BmEm4, a Homologue of Drosophila E(spl)m4, from the Silkworm, Bombyx mori
title_full Characterization of the Gene BmEm4, a Homologue of Drosophila E(spl)m4, from the Silkworm, Bombyx mori
title_fullStr Characterization of the Gene BmEm4, a Homologue of Drosophila E(spl)m4, from the Silkworm, Bombyx mori
title_full_unstemmed Characterization of the Gene BmEm4, a Homologue of Drosophila E(spl)m4, from the Silkworm, Bombyx mori
title_short Characterization of the Gene BmEm4, a Homologue of Drosophila E(spl)m4, from the Silkworm, Bombyx mori
title_sort characterization of the gene bmem4, a homologue of drosophila e(spl)m4, from the silkworm, bombyx mori
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2760746/
https://www.ncbi.nlm.nih.gov/pubmed/19830255
http://dx.doi.org/10.1155/2009/947490
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