In vitro characterization of a miR-122-sensitive double-helical switch element in the 5′ region of hepatitis C virus RNA

It has been proposed that the hepatitis C virus (HCV) internal ribosome entry site (IRES) resides within a locked conformation, owing to annealing of its immediate flanking sequences. In this study, structure probing using Escherichia coli dsRNA-specific RNase III and other classical tools showed th...

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Autores principales: Díaz-Toledano, Rosa, Ariza-Mateos, Ascensión, Birk, Alex, Martínez-García, Belén, Gómez, Jordi
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2760801/
https://www.ncbi.nlm.nih.gov/pubmed/19578061
http://dx.doi.org/10.1093/nar/gkp553
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author Díaz-Toledano, Rosa
Ariza-Mateos, Ascensión
Birk, Alex
Martínez-García, Belén
Gómez, Jordi
author_facet Díaz-Toledano, Rosa
Ariza-Mateos, Ascensión
Birk, Alex
Martínez-García, Belén
Gómez, Jordi
author_sort Díaz-Toledano, Rosa
collection PubMed
description It has been proposed that the hepatitis C virus (HCV) internal ribosome entry site (IRES) resides within a locked conformation, owing to annealing of its immediate flanking sequences. In this study, structure probing using Escherichia coli dsRNA-specific RNase III and other classical tools showed that this region switches to an open conformation triggered by the liver-specific microRNA, miR-122. This structural transition, observed in vitro, may be the mechanistic basis for the involvement of downstream IRES structural domain VI in translation, as well as providing a role of liver-specific miR-122 in HCV infection. In addition, the induced RNA switching at the 5′ untranslated region could ultimately represent a new mechanism of action of micro-RNAs.
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spelling pubmed-27608012009-10-13 In vitro characterization of a miR-122-sensitive double-helical switch element in the 5′ region of hepatitis C virus RNA Díaz-Toledano, Rosa Ariza-Mateos, Ascensión Birk, Alex Martínez-García, Belén Gómez, Jordi Nucleic Acids Res RNA It has been proposed that the hepatitis C virus (HCV) internal ribosome entry site (IRES) resides within a locked conformation, owing to annealing of its immediate flanking sequences. In this study, structure probing using Escherichia coli dsRNA-specific RNase III and other classical tools showed that this region switches to an open conformation triggered by the liver-specific microRNA, miR-122. This structural transition, observed in vitro, may be the mechanistic basis for the involvement of downstream IRES structural domain VI in translation, as well as providing a role of liver-specific miR-122 in HCV infection. In addition, the induced RNA switching at the 5′ untranslated region could ultimately represent a new mechanism of action of micro-RNAs. Oxford University Press 2009-09 2009-07-03 /pmc/articles/PMC2760801/ /pubmed/19578061 http://dx.doi.org/10.1093/nar/gkp553 Text en © 2009 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA
Díaz-Toledano, Rosa
Ariza-Mateos, Ascensión
Birk, Alex
Martínez-García, Belén
Gómez, Jordi
In vitro characterization of a miR-122-sensitive double-helical switch element in the 5′ region of hepatitis C virus RNA
title In vitro characterization of a miR-122-sensitive double-helical switch element in the 5′ region of hepatitis C virus RNA
title_full In vitro characterization of a miR-122-sensitive double-helical switch element in the 5′ region of hepatitis C virus RNA
title_fullStr In vitro characterization of a miR-122-sensitive double-helical switch element in the 5′ region of hepatitis C virus RNA
title_full_unstemmed In vitro characterization of a miR-122-sensitive double-helical switch element in the 5′ region of hepatitis C virus RNA
title_short In vitro characterization of a miR-122-sensitive double-helical switch element in the 5′ region of hepatitis C virus RNA
title_sort in vitro characterization of a mir-122-sensitive double-helical switch element in the 5′ region of hepatitis c virus rna
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2760801/
https://www.ncbi.nlm.nih.gov/pubmed/19578061
http://dx.doi.org/10.1093/nar/gkp553
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