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Studying human telomerase gene transcription by a chromatinized reporter generated by recombinase-mediated targeting of a bacterial artificial chromosome

The endogenous human telomerase reverse transcriptase (hTERT) gene is repressed in somatic cells. To study the mechanisms of its repression, we developed a strategy of retrovirus-directed Cre recombinase-mediated BAC targeting, or RMBT, to generate single-copy integrations of BAC at pre-engineered c...

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Detalles Bibliográficos
Autores principales:	Wang, Shuwen, Zhao, Yuanjun, Leiby, Melanie A., Zhu, Jiyue
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2009
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2761251/ https://www.ncbi.nlm.nih.gov/pubmed/19528078 http://dx.doi.org/10.1093/nar/gkp511

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