Preparation of selective and segmentally labeled single-stranded DNA for NMR by self-primed PCR and asymmetrical endonuclease double digestion

We demonstrate a new, efficient and easy-to-use method for enzymatic synthesis of (stereo-)specific and segmental (13)C/(15)N/(2)H isotope-labeled single-stranded DNA in amounts sufficient for NMR, based on the highly efficient self-primed PCR. To achieve this, new approaches are introduced and comb...

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Detalles Bibliográficos
Autores principales: Nelissen, Frank H. T., Girard, Frederic C., Tessari, Marco, Heus, Hans A., Wijmenga, Sybren S.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2761255/
https://www.ncbi.nlm.nih.gov/pubmed/19553193
http://dx.doi.org/10.1093/nar/gkp540

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2761255/
https://www.ncbi.nlm.nih.gov/pubmed/19553193
http://dx.doi.org/10.1093/nar/gkp540

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