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A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization

We describe a quantitative method for detecting RNA alternative splicing variants that combines in situ hybridization of fluorescently labeled peptide nucleic acid (PNA) probes with confocal microscopy Förster resonance energy transfer (FRET). The use of PNA probes complementary to sequences flankin...

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Detalles Bibliográficos
Autores principales:	Blanco, Ana M., Rausell, Laura, Aguado, Begoña, Perez-Alonso, Manuel, Artero, Rubén
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2009
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2761257/ https://www.ncbi.nlm.nih.gov/pubmed/19561195 http://dx.doi.org/10.1093/nar/gkp551

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