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The expression of PLK-1 in cervical carcinoma: a possible target for enhancing chemosensitivity
BACKGROUND: Polo-like kinase-1 (PLK-1) is reported to be upregulated in a variety of human tumors and is implicated in cell proliferation and survival. However, its importance in cervical carcinoma has not yet been fully elucidated. METHODS: We examined PLK-1 expression in cervical carcinoma tissues...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2761395/ https://www.ncbi.nlm.nih.gov/pubmed/19775446 http://dx.doi.org/10.1186/1756-9966-28-130 |
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author | Zhang, Yuan Liu, Yu Yang, Yuan-Xian Xia, Jia-Hong Zhang, Hong-Xiu Li, Hua-Bin Yu, Chun-Zhao |
author_facet | Zhang, Yuan Liu, Yu Yang, Yuan-Xian Xia, Jia-Hong Zhang, Hong-Xiu Li, Hua-Bin Yu, Chun-Zhao |
author_sort | Zhang, Yuan |
collection | PubMed |
description | BACKGROUND: Polo-like kinase-1 (PLK-1) is reported to be upregulated in a variety of human tumors and is implicated in cell proliferation and survival. However, its importance in cervical carcinoma has not yet been fully elucidated. METHODS: We examined PLK-1 expression in cervical carcinoma tissues using immunohistochemical staining. Furthermore, we blocked PLK-1 expression in HeLa cells using specific siRNA and detected the cell cycle, cell proliferation and chemosensitivity using western blotting, MTT and flow cytometry. RESULTS: We provide evidence that expression of PLK-1 exists in human cervical carcinoma tissues and establish an association with tumor size. Furthermore, we show that PLK-1 knockdown by transfection of siRNA induces accumulation of HeLa cells in the G2/M cell cycle phase and enhances cisplatin-induced apoptosis. CONCLUSION: Our results indicate that PLK-1 production in HeLa cells might be critical in determining whether cells survive or undergo apoptosis. Therefore, targeting PLK-1 might be a promising strategy for enhancing sensitivity to chemotherapeutic reagents in cervical carcinoma. |
format | Text |
id | pubmed-2761395 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-27613952009-10-14 The expression of PLK-1 in cervical carcinoma: a possible target for enhancing chemosensitivity Zhang, Yuan Liu, Yu Yang, Yuan-Xian Xia, Jia-Hong Zhang, Hong-Xiu Li, Hua-Bin Yu, Chun-Zhao J Exp Clin Cancer Res Research BACKGROUND: Polo-like kinase-1 (PLK-1) is reported to be upregulated in a variety of human tumors and is implicated in cell proliferation and survival. However, its importance in cervical carcinoma has not yet been fully elucidated. METHODS: We examined PLK-1 expression in cervical carcinoma tissues using immunohistochemical staining. Furthermore, we blocked PLK-1 expression in HeLa cells using specific siRNA and detected the cell cycle, cell proliferation and chemosensitivity using western blotting, MTT and flow cytometry. RESULTS: We provide evidence that expression of PLK-1 exists in human cervical carcinoma tissues and establish an association with tumor size. Furthermore, we show that PLK-1 knockdown by transfection of siRNA induces accumulation of HeLa cells in the G2/M cell cycle phase and enhances cisplatin-induced apoptosis. CONCLUSION: Our results indicate that PLK-1 production in HeLa cells might be critical in determining whether cells survive or undergo apoptosis. Therefore, targeting PLK-1 might be a promising strategy for enhancing sensitivity to chemotherapeutic reagents in cervical carcinoma. BioMed Central 2009-09-23 /pmc/articles/PMC2761395/ /pubmed/19775446 http://dx.doi.org/10.1186/1756-9966-28-130 Text en Copyright © 2009 Zhang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Zhang, Yuan Liu, Yu Yang, Yuan-Xian Xia, Jia-Hong Zhang, Hong-Xiu Li, Hua-Bin Yu, Chun-Zhao The expression of PLK-1 in cervical carcinoma: a possible target for enhancing chemosensitivity |
title | The expression of PLK-1 in cervical carcinoma: a possible target for enhancing chemosensitivity |
title_full | The expression of PLK-1 in cervical carcinoma: a possible target for enhancing chemosensitivity |
title_fullStr | The expression of PLK-1 in cervical carcinoma: a possible target for enhancing chemosensitivity |
title_full_unstemmed | The expression of PLK-1 in cervical carcinoma: a possible target for enhancing chemosensitivity |
title_short | The expression of PLK-1 in cervical carcinoma: a possible target for enhancing chemosensitivity |
title_sort | expression of plk-1 in cervical carcinoma: a possible target for enhancing chemosensitivity |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2761395/ https://www.ncbi.nlm.nih.gov/pubmed/19775446 http://dx.doi.org/10.1186/1756-9966-28-130 |
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