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An eIF4E-binding protein regulates katanin protein levels in C. elegans embryos
In Caenorhabditis elegans, the MEI-1–katanin microtubule-severing complex is required for meiosis, but must be down-regulated during the transition to embryogenesis to prevent defects in mitosis. A cullin-dependent degradation pathway for MEI-1 protein has been well documented. In this paper, we rep...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2762092/ https://www.ncbi.nlm.nih.gov/pubmed/19786575 http://dx.doi.org/10.1083/jcb.200903003 |
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author | Li, Wei DeBella, Leah R. Guven-Ozkan, Tugba Lin, Rueyling Rose, Lesilee S. |
author_facet | Li, Wei DeBella, Leah R. Guven-Ozkan, Tugba Lin, Rueyling Rose, Lesilee S. |
author_sort | Li, Wei |
collection | PubMed |
description | In Caenorhabditis elegans, the MEI-1–katanin microtubule-severing complex is required for meiosis, but must be down-regulated during the transition to embryogenesis to prevent defects in mitosis. A cullin-dependent degradation pathway for MEI-1 protein has been well documented. In this paper, we report that translational repression may also play a role in MEI-1 down-regulation. Reduction of spn-2 function results in spindle orientation defects due to ectopic MEI-1 expression during embryonic mitosis. MEL-26, which is both required for MEI-1 degradation and is itself a target of the cullin degradation pathway, is present at normal levels in spn-2 mutant embryos, suggesting that the degradation pathway is functional. Cloning of spn-2 reveals that it encodes an eIF4E-binding protein that localizes to the cytoplasm and to ribonucleoprotein particles called P granules. SPN-2 binds to the RNA-binding protein OMA-1, which in turn binds to the mei-1 3′ untranslated region. Thus, our results suggest that SPN-2 functions as an eIF4E-binding protein to negatively regulate translation of mei-1. |
format | Text |
id | pubmed-2762092 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-27620922010-04-05 An eIF4E-binding protein regulates katanin protein levels in C. elegans embryos Li, Wei DeBella, Leah R. Guven-Ozkan, Tugba Lin, Rueyling Rose, Lesilee S. J Cell Biol Research Articles In Caenorhabditis elegans, the MEI-1–katanin microtubule-severing complex is required for meiosis, but must be down-regulated during the transition to embryogenesis to prevent defects in mitosis. A cullin-dependent degradation pathway for MEI-1 protein has been well documented. In this paper, we report that translational repression may also play a role in MEI-1 down-regulation. Reduction of spn-2 function results in spindle orientation defects due to ectopic MEI-1 expression during embryonic mitosis. MEL-26, which is both required for MEI-1 degradation and is itself a target of the cullin degradation pathway, is present at normal levels in spn-2 mutant embryos, suggesting that the degradation pathway is functional. Cloning of spn-2 reveals that it encodes an eIF4E-binding protein that localizes to the cytoplasm and to ribonucleoprotein particles called P granules. SPN-2 binds to the RNA-binding protein OMA-1, which in turn binds to the mei-1 3′ untranslated region. Thus, our results suggest that SPN-2 functions as an eIF4E-binding protein to negatively regulate translation of mei-1. The Rockefeller University Press 2009-10-05 /pmc/articles/PMC2762092/ /pubmed/19786575 http://dx.doi.org/10.1083/jcb.200903003 Text en © 2009 Li et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Li, Wei DeBella, Leah R. Guven-Ozkan, Tugba Lin, Rueyling Rose, Lesilee S. An eIF4E-binding protein regulates katanin protein levels in C. elegans embryos |
title | An eIF4E-binding protein regulates katanin protein levels in C. elegans embryos |
title_full | An eIF4E-binding protein regulates katanin protein levels in C. elegans embryos |
title_fullStr | An eIF4E-binding protein regulates katanin protein levels in C. elegans embryos |
title_full_unstemmed | An eIF4E-binding protein regulates katanin protein levels in C. elegans embryos |
title_short | An eIF4E-binding protein regulates katanin protein levels in C. elegans embryos |
title_sort | eif4e-binding protein regulates katanin protein levels in c. elegans embryos |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2762092/ https://www.ncbi.nlm.nih.gov/pubmed/19786575 http://dx.doi.org/10.1083/jcb.200903003 |
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