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Cdc42 antagonizes Rho1 activity at adherens junctions to limit epithelial cell apical tension

In epithelia, cells are arranged in an orderly pattern with a defined orientation and shape. Cadherin containing apical adherens junctions (AJs) and the associated actomyosin cytoskeleton likely contribute to epithelial cell shape by providing apical tension. The Rho guanosine triphosphatases are we...

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Detalles Bibliográficos
Autores principales: Warner, Stephen J., Longmore, Gregory D.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2762093/
https://www.ncbi.nlm.nih.gov/pubmed/19805632
http://dx.doi.org/10.1083/jcb.200906047
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author Warner, Stephen J.
Longmore, Gregory D.
author_facet Warner, Stephen J.
Longmore, Gregory D.
author_sort Warner, Stephen J.
collection PubMed
description In epithelia, cells are arranged in an orderly pattern with a defined orientation and shape. Cadherin containing apical adherens junctions (AJs) and the associated actomyosin cytoskeleton likely contribute to epithelial cell shape by providing apical tension. The Rho guanosine triphosphatases are well known regulators of cell junction formation, maintenance, and function. Specifically, Rho promotes actomyosin activity and cell contractility; however, what controls and localizes this Rho activity as epithelia remodel is unresolved. Using mosaic clonal analysis in the Drosophila melanogaster pupal eye, we find that Cdc42 is critical for limiting apical cell tension by antagonizing Rho activity at AJs. Cdc42 localizes Par6–atypical protein kinase C (aPKC) to AJs, where this complex limits Rho1 activity and thus actomyosin contractility, independent of its effects on Wiskott-Aldrich syndrome protein and p21-activated kinase. Thus, in addition to its role in the establishment and maintenance of apical–basal polarity in forming epithelia, the Cdc42–Par6–aPKC polarity complex is required to limit Rho activity at AJs and thus modulate apical tension so as to shape the final epithelium.
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spelling pubmed-27620932010-04-05 Cdc42 antagonizes Rho1 activity at adherens junctions to limit epithelial cell apical tension Warner, Stephen J. Longmore, Gregory D. J Cell Biol Research Articles In epithelia, cells are arranged in an orderly pattern with a defined orientation and shape. Cadherin containing apical adherens junctions (AJs) and the associated actomyosin cytoskeleton likely contribute to epithelial cell shape by providing apical tension. The Rho guanosine triphosphatases are well known regulators of cell junction formation, maintenance, and function. Specifically, Rho promotes actomyosin activity and cell contractility; however, what controls and localizes this Rho activity as epithelia remodel is unresolved. Using mosaic clonal analysis in the Drosophila melanogaster pupal eye, we find that Cdc42 is critical for limiting apical cell tension by antagonizing Rho activity at AJs. Cdc42 localizes Par6–atypical protein kinase C (aPKC) to AJs, where this complex limits Rho1 activity and thus actomyosin contractility, independent of its effects on Wiskott-Aldrich syndrome protein and p21-activated kinase. Thus, in addition to its role in the establishment and maintenance of apical–basal polarity in forming epithelia, the Cdc42–Par6–aPKC polarity complex is required to limit Rho activity at AJs and thus modulate apical tension so as to shape the final epithelium. The Rockefeller University Press 2009-10-05 /pmc/articles/PMC2762093/ /pubmed/19805632 http://dx.doi.org/10.1083/jcb.200906047 Text en © 2009 Warner and Longmore This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Warner, Stephen J.
Longmore, Gregory D.
Cdc42 antagonizes Rho1 activity at adherens junctions to limit epithelial cell apical tension
title Cdc42 antagonizes Rho1 activity at adherens junctions to limit epithelial cell apical tension
title_full Cdc42 antagonizes Rho1 activity at adherens junctions to limit epithelial cell apical tension
title_fullStr Cdc42 antagonizes Rho1 activity at adherens junctions to limit epithelial cell apical tension
title_full_unstemmed Cdc42 antagonizes Rho1 activity at adherens junctions to limit epithelial cell apical tension
title_short Cdc42 antagonizes Rho1 activity at adherens junctions to limit epithelial cell apical tension
title_sort cdc42 antagonizes rho1 activity at adherens junctions to limit epithelial cell apical tension
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2762093/
https://www.ncbi.nlm.nih.gov/pubmed/19805632
http://dx.doi.org/10.1083/jcb.200906047
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