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Autophagy promotes synapse development in Drosophila
Autophagy, a lysosome-dependent degradation mechanism, mediates many biological processes, including cellular stress responses and neuroprotection. In this study, we demonstrate that autophagy positively regulates development of the Drosophila melanogaster larval neuromuscular junction (NMJ). Autoph...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2762098/ https://www.ncbi.nlm.nih.gov/pubmed/19786572 http://dx.doi.org/10.1083/jcb.200907109 |
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author | Shen, Wei Ganetzky, Barry |
author_facet | Shen, Wei Ganetzky, Barry |
author_sort | Shen, Wei |
collection | PubMed |
description | Autophagy, a lysosome-dependent degradation mechanism, mediates many biological processes, including cellular stress responses and neuroprotection. In this study, we demonstrate that autophagy positively regulates development of the Drosophila melanogaster larval neuromuscular junction (NMJ). Autophagy induces an NMJ overgrowth phenotype closely resembling that of highwire (hiw), an E3 ubiquitin ligase mutant. Moreover, like hiw, autophagy-induced NMJ overgrowth is suppressed by wallenda (wnd) and by a dominant-negative c-Jun NH(2)-terminal kinase (bsk(DN)). We show that autophagy promotes NMJ growth by reducing Hiw levels. Thus, autophagy and the ubiquitin–proteasome system converge in regulating synaptic development. Because autophagy is triggered in response to many environmental cues, our findings suggest that it is perfectly positioned to link environmental conditions with synaptic growth and plasticity. |
format | Text |
id | pubmed-2762098 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-27620982010-04-05 Autophagy promotes synapse development in Drosophila Shen, Wei Ganetzky, Barry J Cell Biol Research Articles Autophagy, a lysosome-dependent degradation mechanism, mediates many biological processes, including cellular stress responses and neuroprotection. In this study, we demonstrate that autophagy positively regulates development of the Drosophila melanogaster larval neuromuscular junction (NMJ). Autophagy induces an NMJ overgrowth phenotype closely resembling that of highwire (hiw), an E3 ubiquitin ligase mutant. Moreover, like hiw, autophagy-induced NMJ overgrowth is suppressed by wallenda (wnd) and by a dominant-negative c-Jun NH(2)-terminal kinase (bsk(DN)). We show that autophagy promotes NMJ growth by reducing Hiw levels. Thus, autophagy and the ubiquitin–proteasome system converge in regulating synaptic development. Because autophagy is triggered in response to many environmental cues, our findings suggest that it is perfectly positioned to link environmental conditions with synaptic growth and plasticity. The Rockefeller University Press 2009-10-05 /pmc/articles/PMC2762098/ /pubmed/19786572 http://dx.doi.org/10.1083/jcb.200907109 Text en © 2009 Shen and Ganetzky This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Shen, Wei Ganetzky, Barry Autophagy promotes synapse development in Drosophila |
title | Autophagy promotes synapse development in Drosophila |
title_full | Autophagy promotes synapse development in Drosophila |
title_fullStr | Autophagy promotes synapse development in Drosophila |
title_full_unstemmed | Autophagy promotes synapse development in Drosophila |
title_short | Autophagy promotes synapse development in Drosophila |
title_sort | autophagy promotes synapse development in drosophila |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2762098/ https://www.ncbi.nlm.nih.gov/pubmed/19786572 http://dx.doi.org/10.1083/jcb.200907109 |
work_keys_str_mv | AT shenwei autophagypromotessynapsedevelopmentindrosophila AT ganetzkybarry autophagypromotessynapsedevelopmentindrosophila |