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Chenopodium album prevents progression of cell growth and enhances cell toxicity in human breast cancer cell lines
The present study is aimed to investigate the effects of Chenopodium album (leaves) on the growth of estrogen dependent (MCF-7) and estrogen independent (MDA-MB-468) human breast cancer cell lines. The different solvent extracts (petroleum ether, ethyl acetate and methanol) were assessed for their c...
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Formato: | Texto |
Lenguaje: | English |
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Landes Bioscience
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2763242/ https://www.ncbi.nlm.nih.gov/pubmed/20592771 |
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author | Khoobchandani, Menka Ojeswi, BK Sharma, Bhavna Srivastava, Man Mohan |
author_facet | Khoobchandani, Menka Ojeswi, BK Sharma, Bhavna Srivastava, Man Mohan |
author_sort | Khoobchandani, Menka |
collection | PubMed |
description | The present study is aimed to investigate the effects of Chenopodium album (leaves) on the growth of estrogen dependent (MCF-7) and estrogen independent (MDA-MB-468) human breast cancer cell lines. The different solvent extracts (petroleum ether, ethyl acetate and methanol) were assessed for their cytotoxicity using TBE (Trypan blue exclusion) and MTT [3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium] bioassay. These cells were cultured in MEM (minimum essential medium) medium and incubated with the dilution series of extracts (10–100 mg/ml) in CO(2) incubator at 37°C for 24 h. Among the various extracts studied for two cell lines, methanolic extract of C. album (leaves) exhibited maximum antibreast cancer activity having IC(50) (the concentration of an individual compound leading to 50% inhibition) value 27.31 mg/ml against MCF-7 cell line. Significant percent inhibition (94.06%) in the MeOH extract of C. album (leaves) at 48 h of exposure and concentration 100 mg/ml (p < 0.05) against MCF-7 breast cancer cell line, indicates the presence of some structural moiety responsible for this observed antiproliferative effect. In vivo study and structural elucidation of its bioactive principle are in progress. Our findings highlight the potential of this plant for its possible clinical use to counteract malignancy development as antibreast cancer bioagent. |
format | Text |
id | pubmed-2763242 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Landes Bioscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-27632422010-07-01 Chenopodium album prevents progression of cell growth and enhances cell toxicity in human breast cancer cell lines Khoobchandani, Menka Ojeswi, BK Sharma, Bhavna Srivastava, Man Mohan Oxid Med Cell Longev Research Papers The present study is aimed to investigate the effects of Chenopodium album (leaves) on the growth of estrogen dependent (MCF-7) and estrogen independent (MDA-MB-468) human breast cancer cell lines. The different solvent extracts (petroleum ether, ethyl acetate and methanol) were assessed for their cytotoxicity using TBE (Trypan blue exclusion) and MTT [3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium] bioassay. These cells were cultured in MEM (minimum essential medium) medium and incubated with the dilution series of extracts (10–100 mg/ml) in CO(2) incubator at 37°C for 24 h. Among the various extracts studied for two cell lines, methanolic extract of C. album (leaves) exhibited maximum antibreast cancer activity having IC(50) (the concentration of an individual compound leading to 50% inhibition) value 27.31 mg/ml against MCF-7 cell line. Significant percent inhibition (94.06%) in the MeOH extract of C. album (leaves) at 48 h of exposure and concentration 100 mg/ml (p < 0.05) against MCF-7 breast cancer cell line, indicates the presence of some structural moiety responsible for this observed antiproliferative effect. In vivo study and structural elucidation of its bioactive principle are in progress. Our findings highlight the potential of this plant for its possible clinical use to counteract malignancy development as antibreast cancer bioagent. Landes Bioscience 2009 /pmc/articles/PMC2763242/ /pubmed/20592771 Text en Copyright © 2009 Landes Bioscience |
spellingShingle | Research Papers Khoobchandani, Menka Ojeswi, BK Sharma, Bhavna Srivastava, Man Mohan Chenopodium album prevents progression of cell growth and enhances cell toxicity in human breast cancer cell lines |
title | Chenopodium album prevents progression of cell growth and enhances cell toxicity in human breast cancer cell lines |
title_full | Chenopodium album prevents progression of cell growth and enhances cell toxicity in human breast cancer cell lines |
title_fullStr | Chenopodium album prevents progression of cell growth and enhances cell toxicity in human breast cancer cell lines |
title_full_unstemmed | Chenopodium album prevents progression of cell growth and enhances cell toxicity in human breast cancer cell lines |
title_short | Chenopodium album prevents progression of cell growth and enhances cell toxicity in human breast cancer cell lines |
title_sort | chenopodium album prevents progression of cell growth and enhances cell toxicity in human breast cancer cell lines |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2763242/ https://www.ncbi.nlm.nih.gov/pubmed/20592771 |
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