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The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
It is becoming increasingly apparent that electroporation is the most effective way to introduce plasmid DNA or siRNA into primary cells. The Gene Pulser MXcell electroporation system and Gene Pulser electroporation buffer were specifically developed to transfect nucleic acids into mammalian cells a...
Autores principales: | Kroeger, Kelly, Collins, Michelle, Ugozzoli, Luis |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2763291/ https://www.ncbi.nlm.nih.gov/pubmed/19229174 http://dx.doi.org/10.3791/1026 |
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