High-quality genomic DNA extraction from formalin-fixed and paraffin-embedded samples deparaffinized using mineral oil

Extracting DNA from formalin-fixed and paraffin-embedded (FFPE) tissue remains a challenge, despite numerous attempts to develop a more effective method. Polymerase chain reaction (PCR) success rates with DNA extracted using current methods remain low. We extracted DNA from 140 long-term archived FF...

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Detalles Bibliográficos
Autores principales: Lin, Jianghai, Kennedy, Stephen H., Svarovsky, Therese, Rogers, Jeffrey, Kemnitz, Joseph W., Xu, Anlong, Zondervan, Krina T.
Formato: Texto
Lenguaje:English
Publicado: Academic Press 2009
Materias:
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2764035/
https://www.ncbi.nlm.nih.gov/pubmed/19698695
http://dx.doi.org/10.1016/j.ab.2009.08.016
Descripción
Sumario:Extracting DNA from formalin-fixed and paraffin-embedded (FFPE) tissue remains a challenge, despite numerous attempts to develop a more effective method. Polymerase chain reaction (PCR) success rates with DNA extracted using current methods remain low. We extracted DNA from 140 long-term archived FFPE samples using a simple but effective deparaffinization method, removing the wax with mineral oil, and a commercially available DNA extraction kit. DNA quality was subsequently tested in a genotyping experiment with 14 microsatellite markers. High-quality DNA was obtained with a mean PCR success rate of 97% (range: 88–100%) across markers. The results suggested that DNA extracted using this novel method is likely to be suitable for genetic studies involving DNA fragments <200 bp.

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