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Stm1p alters the ribosome association of eukaryotic elongation factor 3 and affects translation elongation
Stm1p is a Saccharomyces cerevisiae protein that is primarily associated with cytosolic 80S ribosomes and polysomes. Several lines of evidence suggest that Stm1p plays a role in translation under nutrient stress conditions, although its mechanism of action is not yet known. In this study, we show th...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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Oxford University Press
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2764444/ https://www.ncbi.nlm.nih.gov/pubmed/19666721 http://dx.doi.org/10.1093/nar/gkp645 |
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author | Van Dyke, Natalya Pickering, Brian F. Van Dyke, Michael W. |
author_facet | Van Dyke, Natalya Pickering, Brian F. Van Dyke, Michael W. |
author_sort | Van Dyke, Natalya |
collection | PubMed |
description | Stm1p is a Saccharomyces cerevisiae protein that is primarily associated with cytosolic 80S ribosomes and polysomes. Several lines of evidence suggest that Stm1p plays a role in translation under nutrient stress conditions, although its mechanism of action is not yet known. In this study, we show that yeast lacking Stm1p (stm1Δ) are hypersensitive to the translation inhibitor anisomycin, which affects the peptidyl transferase reaction in translation elongation, but show little hypersensitivity to other translation inhibitors such as paromomycin and hygromycin B, which affect translation fidelity. Ribosomes isolated from stm1Δ yeast have intrinsically elevated levels of eukaryotic elongation factor 3 (eEF3) associated with them. Overexpression of eEF3 in cells lacking Stm1p results in a growth defect phenotype and increased anisomycin sensitivity. In addition, ribosomes with increased levels of Stm1p exhibit decreased association with eEF3. Taken together, our data indicate that Stm1p plays a complementary role to eEF3 in translation. |
format | Text |
id | pubmed-2764444 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-27644442009-10-20 Stm1p alters the ribosome association of eukaryotic elongation factor 3 and affects translation elongation Van Dyke, Natalya Pickering, Brian F. Van Dyke, Michael W. Nucleic Acids Res Molecular Biology Stm1p is a Saccharomyces cerevisiae protein that is primarily associated with cytosolic 80S ribosomes and polysomes. Several lines of evidence suggest that Stm1p plays a role in translation under nutrient stress conditions, although its mechanism of action is not yet known. In this study, we show that yeast lacking Stm1p (stm1Δ) are hypersensitive to the translation inhibitor anisomycin, which affects the peptidyl transferase reaction in translation elongation, but show little hypersensitivity to other translation inhibitors such as paromomycin and hygromycin B, which affect translation fidelity. Ribosomes isolated from stm1Δ yeast have intrinsically elevated levels of eukaryotic elongation factor 3 (eEF3) associated with them. Overexpression of eEF3 in cells lacking Stm1p results in a growth defect phenotype and increased anisomycin sensitivity. In addition, ribosomes with increased levels of Stm1p exhibit decreased association with eEF3. Taken together, our data indicate that Stm1p plays a complementary role to eEF3 in translation. Oxford University Press 2009-10 2009-08-07 /pmc/articles/PMC2764444/ /pubmed/19666721 http://dx.doi.org/10.1093/nar/gkp645 Text en © 2009 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Molecular Biology Van Dyke, Natalya Pickering, Brian F. Van Dyke, Michael W. Stm1p alters the ribosome association of eukaryotic elongation factor 3 and affects translation elongation |
title | Stm1p alters the ribosome association of eukaryotic elongation factor 3 and affects translation elongation |
title_full | Stm1p alters the ribosome association of eukaryotic elongation factor 3 and affects translation elongation |
title_fullStr | Stm1p alters the ribosome association of eukaryotic elongation factor 3 and affects translation elongation |
title_full_unstemmed | Stm1p alters the ribosome association of eukaryotic elongation factor 3 and affects translation elongation |
title_short | Stm1p alters the ribosome association of eukaryotic elongation factor 3 and affects translation elongation |
title_sort | stm1p alters the ribosome association of eukaryotic elongation factor 3 and affects translation elongation |
topic | Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2764444/ https://www.ncbi.nlm.nih.gov/pubmed/19666721 http://dx.doi.org/10.1093/nar/gkp645 |
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