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Genome Sequence of the Versatile Fish Pathogen Edwardsiella tarda Provides Insights into its Adaptation to Broad Host Ranges and Intracellular Niches

BACKGROUND: Edwardsiella tarda is the etiologic agent of edwardsiellosis, a devastating fish disease prevailing in worldwide aquaculture industries. Here we describe the complete genome of E. tarda, EIB202, a highly virulent and multi-drug resistant isolate in China. METHODOLOGY/PRINCIPAL FINDINGS:...

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Autores principales: Wang, Qiyao, Yang, Minjun, Xiao, Jingfan, Wu, Haizhen, Wang, Xin, Lv, Yuanzhi, Xu, Lili, Zheng, Huajun, Wang, Shengyue, Zhao, Guoping, Liu, Qin, Zhang, Yuanxing
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2764856/
https://www.ncbi.nlm.nih.gov/pubmed/19865481
http://dx.doi.org/10.1371/journal.pone.0007646
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author Wang, Qiyao
Yang, Minjun
Xiao, Jingfan
Wu, Haizhen
Wang, Xin
Lv, Yuanzhi
Xu, Lili
Zheng, Huajun
Wang, Shengyue
Zhao, Guoping
Liu, Qin
Zhang, Yuanxing
author_facet Wang, Qiyao
Yang, Minjun
Xiao, Jingfan
Wu, Haizhen
Wang, Xin
Lv, Yuanzhi
Xu, Lili
Zheng, Huajun
Wang, Shengyue
Zhao, Guoping
Liu, Qin
Zhang, Yuanxing
author_sort Wang, Qiyao
collection PubMed
description BACKGROUND: Edwardsiella tarda is the etiologic agent of edwardsiellosis, a devastating fish disease prevailing in worldwide aquaculture industries. Here we describe the complete genome of E. tarda, EIB202, a highly virulent and multi-drug resistant isolate in China. METHODOLOGY/PRINCIPAL FINDINGS: E. tarda EIB202 possesses a single chromosome of 3,760,463 base pairs containing 3,486 predicted protein coding sequences, 8 ribosomal rRNA operons, and 95 tRNA genes, and a 43,703 bp conjugative plasmid harboring multi-drug resistant determinants and encoding type IV A secretion system components. We identified a full spectrum of genetic properties related to its genome plasticity such as repeated sequences, insertion sequences, phage-like proteins, integrases, recombinases and genomic islands. In addition, analysis also indicated that a substantial proportion of the E. tarda genome might be devoted to the growth and survival under diverse conditions including intracellular niches, with a large number of aerobic or anaerobic respiration-associated proteins, signal transduction proteins as well as proteins involved in various stress adaptations. A pool of genes for secretion systems, pili formation, nonfimbrial adhesions, invasions and hemagglutinins, chondroitinases, hemolysins, iron scavenging systems as well as the incomplete flagellar biogenesis might feature its surface structures and pathogenesis in a fish body. CONCLUSION/SIGNIFICANCE: Genomic analysis of the bacterium offered insights into the phylogeny, metabolism, drug-resistance, stress adaptation, and virulence characteristics of this versatile pathogen, which constitutes an important first step in understanding the pathogenesis of E. tarda to facilitate construction of a practical effective vaccine used for combating fish edwardsiellosis.
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spelling pubmed-27648562009-10-29 Genome Sequence of the Versatile Fish Pathogen Edwardsiella tarda Provides Insights into its Adaptation to Broad Host Ranges and Intracellular Niches Wang, Qiyao Yang, Minjun Xiao, Jingfan Wu, Haizhen Wang, Xin Lv, Yuanzhi Xu, Lili Zheng, Huajun Wang, Shengyue Zhao, Guoping Liu, Qin Zhang, Yuanxing PLoS One Research Article BACKGROUND: Edwardsiella tarda is the etiologic agent of edwardsiellosis, a devastating fish disease prevailing in worldwide aquaculture industries. Here we describe the complete genome of E. tarda, EIB202, a highly virulent and multi-drug resistant isolate in China. METHODOLOGY/PRINCIPAL FINDINGS: E. tarda EIB202 possesses a single chromosome of 3,760,463 base pairs containing 3,486 predicted protein coding sequences, 8 ribosomal rRNA operons, and 95 tRNA genes, and a 43,703 bp conjugative plasmid harboring multi-drug resistant determinants and encoding type IV A secretion system components. We identified a full spectrum of genetic properties related to its genome plasticity such as repeated sequences, insertion sequences, phage-like proteins, integrases, recombinases and genomic islands. In addition, analysis also indicated that a substantial proportion of the E. tarda genome might be devoted to the growth and survival under diverse conditions including intracellular niches, with a large number of aerobic or anaerobic respiration-associated proteins, signal transduction proteins as well as proteins involved in various stress adaptations. A pool of genes for secretion systems, pili formation, nonfimbrial adhesions, invasions and hemagglutinins, chondroitinases, hemolysins, iron scavenging systems as well as the incomplete flagellar biogenesis might feature its surface structures and pathogenesis in a fish body. CONCLUSION/SIGNIFICANCE: Genomic analysis of the bacterium offered insights into the phylogeny, metabolism, drug-resistance, stress adaptation, and virulence characteristics of this versatile pathogen, which constitutes an important first step in understanding the pathogenesis of E. tarda to facilitate construction of a practical effective vaccine used for combating fish edwardsiellosis. Public Library of Science 2009-10-29 /pmc/articles/PMC2764856/ /pubmed/19865481 http://dx.doi.org/10.1371/journal.pone.0007646 Text en Wang et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wang, Qiyao
Yang, Minjun
Xiao, Jingfan
Wu, Haizhen
Wang, Xin
Lv, Yuanzhi
Xu, Lili
Zheng, Huajun
Wang, Shengyue
Zhao, Guoping
Liu, Qin
Zhang, Yuanxing
Genome Sequence of the Versatile Fish Pathogen Edwardsiella tarda Provides Insights into its Adaptation to Broad Host Ranges and Intracellular Niches
title Genome Sequence of the Versatile Fish Pathogen Edwardsiella tarda Provides Insights into its Adaptation to Broad Host Ranges and Intracellular Niches
title_full Genome Sequence of the Versatile Fish Pathogen Edwardsiella tarda Provides Insights into its Adaptation to Broad Host Ranges and Intracellular Niches
title_fullStr Genome Sequence of the Versatile Fish Pathogen Edwardsiella tarda Provides Insights into its Adaptation to Broad Host Ranges and Intracellular Niches
title_full_unstemmed Genome Sequence of the Versatile Fish Pathogen Edwardsiella tarda Provides Insights into its Adaptation to Broad Host Ranges and Intracellular Niches
title_short Genome Sequence of the Versatile Fish Pathogen Edwardsiella tarda Provides Insights into its Adaptation to Broad Host Ranges and Intracellular Niches
title_sort genome sequence of the versatile fish pathogen edwardsiella tarda provides insights into its adaptation to broad host ranges and intracellular niches
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2764856/
https://www.ncbi.nlm.nih.gov/pubmed/19865481
http://dx.doi.org/10.1371/journal.pone.0007646
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