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Alterations in integrin expression modulates invasion of pancreatic cancer cells
BACKGROUND: Factors mediating the invasion of pancreatic cancer cells through the extracellular matrix (ECM) are not fully understood. METHODS: In this study, sub-populations of the human pancreatic cancer cell line, MiaPaCa-2 were established which displayed differences in invasion, adhesion, anoik...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2765436/ https://www.ncbi.nlm.nih.gov/pubmed/19825166 http://dx.doi.org/10.1186/1756-9966-28-140 |
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author | Walsh, Naomi Clynes, Martin Crown, John O'Donovan, Norma |
author_facet | Walsh, Naomi Clynes, Martin Crown, John O'Donovan, Norma |
author_sort | Walsh, Naomi |
collection | PubMed |
description | BACKGROUND: Factors mediating the invasion of pancreatic cancer cells through the extracellular matrix (ECM) are not fully understood. METHODS: In this study, sub-populations of the human pancreatic cancer cell line, MiaPaCa-2 were established which displayed differences in invasion, adhesion, anoikis, anchorage-independent growth and integrin expression. RESULTS: Clone #3 displayed higher invasion with less adhesion, while Clone #8 was less invasive with increased adhesion to ECM proteins compared to MiaPaCa-2. Clone #8 was more sensitive to anoikis than Clone #3 and MiaPaCa-2, and displayed low colony-forming efficiency in an anchorage-independent growth assay. Integrins beta 1, alpha 5 and alpha 6 were over-expressed in Clone #8. Using small interfering RNA (siRNA), integrin β1 knockdown in Clone #8 cells increased invasion through matrigel and fibronectin, increased motility, decreased adhesion and anoikis. Integrin alpha 5 and alpha 6 knockdown also resulted in increased motility, invasion through matrigel and decreased adhesion. CONCLUSION: Our results suggest that altered expression of integrins interacting with different extracellular matrixes may play a significant role in suppressing the aggressive invasive phenotype. Analysis of these clonal populations of MiaPaCa-2 provides a model for investigations into the invasive properties of pancreatic carcinoma. |
format | Text |
id | pubmed-2765436 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-27654362009-10-22 Alterations in integrin expression modulates invasion of pancreatic cancer cells Walsh, Naomi Clynes, Martin Crown, John O'Donovan, Norma J Exp Clin Cancer Res Research BACKGROUND: Factors mediating the invasion of pancreatic cancer cells through the extracellular matrix (ECM) are not fully understood. METHODS: In this study, sub-populations of the human pancreatic cancer cell line, MiaPaCa-2 were established which displayed differences in invasion, adhesion, anoikis, anchorage-independent growth and integrin expression. RESULTS: Clone #3 displayed higher invasion with less adhesion, while Clone #8 was less invasive with increased adhesion to ECM proteins compared to MiaPaCa-2. Clone #8 was more sensitive to anoikis than Clone #3 and MiaPaCa-2, and displayed low colony-forming efficiency in an anchorage-independent growth assay. Integrins beta 1, alpha 5 and alpha 6 were over-expressed in Clone #8. Using small interfering RNA (siRNA), integrin β1 knockdown in Clone #8 cells increased invasion through matrigel and fibronectin, increased motility, decreased adhesion and anoikis. Integrin alpha 5 and alpha 6 knockdown also resulted in increased motility, invasion through matrigel and decreased adhesion. CONCLUSION: Our results suggest that altered expression of integrins interacting with different extracellular matrixes may play a significant role in suppressing the aggressive invasive phenotype. Analysis of these clonal populations of MiaPaCa-2 provides a model for investigations into the invasive properties of pancreatic carcinoma. BioMed Central 2009-10-13 /pmc/articles/PMC2765436/ /pubmed/19825166 http://dx.doi.org/10.1186/1756-9966-28-140 Text en Copyright © 2009 Walsh et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Walsh, Naomi Clynes, Martin Crown, John O'Donovan, Norma Alterations in integrin expression modulates invasion of pancreatic cancer cells |
title | Alterations in integrin expression modulates invasion of pancreatic cancer cells |
title_full | Alterations in integrin expression modulates invasion of pancreatic cancer cells |
title_fullStr | Alterations in integrin expression modulates invasion of pancreatic cancer cells |
title_full_unstemmed | Alterations in integrin expression modulates invasion of pancreatic cancer cells |
title_short | Alterations in integrin expression modulates invasion of pancreatic cancer cells |
title_sort | alterations in integrin expression modulates invasion of pancreatic cancer cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2765436/ https://www.ncbi.nlm.nih.gov/pubmed/19825166 http://dx.doi.org/10.1186/1756-9966-28-140 |
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