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Alterations in integrin expression modulates invasion of pancreatic cancer cells

BACKGROUND: Factors mediating the invasion of pancreatic cancer cells through the extracellular matrix (ECM) are not fully understood. METHODS: In this study, sub-populations of the human pancreatic cancer cell line, MiaPaCa-2 were established which displayed differences in invasion, adhesion, anoik...

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Detalles Bibliográficos
Autores principales: Walsh, Naomi, Clynes, Martin, Crown, John, O'Donovan, Norma
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2765436/
https://www.ncbi.nlm.nih.gov/pubmed/19825166
http://dx.doi.org/10.1186/1756-9966-28-140
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author Walsh, Naomi
Clynes, Martin
Crown, John
O'Donovan, Norma
author_facet Walsh, Naomi
Clynes, Martin
Crown, John
O'Donovan, Norma
author_sort Walsh, Naomi
collection PubMed
description BACKGROUND: Factors mediating the invasion of pancreatic cancer cells through the extracellular matrix (ECM) are not fully understood. METHODS: In this study, sub-populations of the human pancreatic cancer cell line, MiaPaCa-2 were established which displayed differences in invasion, adhesion, anoikis, anchorage-independent growth and integrin expression. RESULTS: Clone #3 displayed higher invasion with less adhesion, while Clone #8 was less invasive with increased adhesion to ECM proteins compared to MiaPaCa-2. Clone #8 was more sensitive to anoikis than Clone #3 and MiaPaCa-2, and displayed low colony-forming efficiency in an anchorage-independent growth assay. Integrins beta 1, alpha 5 and alpha 6 were over-expressed in Clone #8. Using small interfering RNA (siRNA), integrin β1 knockdown in Clone #8 cells increased invasion through matrigel and fibronectin, increased motility, decreased adhesion and anoikis. Integrin alpha 5 and alpha 6 knockdown also resulted in increased motility, invasion through matrigel and decreased adhesion. CONCLUSION: Our results suggest that altered expression of integrins interacting with different extracellular matrixes may play a significant role in suppressing the aggressive invasive phenotype. Analysis of these clonal populations of MiaPaCa-2 provides a model for investigations into the invasive properties of pancreatic carcinoma.
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spelling pubmed-27654362009-10-22 Alterations in integrin expression modulates invasion of pancreatic cancer cells Walsh, Naomi Clynes, Martin Crown, John O'Donovan, Norma J Exp Clin Cancer Res Research BACKGROUND: Factors mediating the invasion of pancreatic cancer cells through the extracellular matrix (ECM) are not fully understood. METHODS: In this study, sub-populations of the human pancreatic cancer cell line, MiaPaCa-2 were established which displayed differences in invasion, adhesion, anoikis, anchorage-independent growth and integrin expression. RESULTS: Clone #3 displayed higher invasion with less adhesion, while Clone #8 was less invasive with increased adhesion to ECM proteins compared to MiaPaCa-2. Clone #8 was more sensitive to anoikis than Clone #3 and MiaPaCa-2, and displayed low colony-forming efficiency in an anchorage-independent growth assay. Integrins beta 1, alpha 5 and alpha 6 were over-expressed in Clone #8. Using small interfering RNA (siRNA), integrin β1 knockdown in Clone #8 cells increased invasion through matrigel and fibronectin, increased motility, decreased adhesion and anoikis. Integrin alpha 5 and alpha 6 knockdown also resulted in increased motility, invasion through matrigel and decreased adhesion. CONCLUSION: Our results suggest that altered expression of integrins interacting with different extracellular matrixes may play a significant role in suppressing the aggressive invasive phenotype. Analysis of these clonal populations of MiaPaCa-2 provides a model for investigations into the invasive properties of pancreatic carcinoma. BioMed Central 2009-10-13 /pmc/articles/PMC2765436/ /pubmed/19825166 http://dx.doi.org/10.1186/1756-9966-28-140 Text en Copyright © 2009 Walsh et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Walsh, Naomi
Clynes, Martin
Crown, John
O'Donovan, Norma
Alterations in integrin expression modulates invasion of pancreatic cancer cells
title Alterations in integrin expression modulates invasion of pancreatic cancer cells
title_full Alterations in integrin expression modulates invasion of pancreatic cancer cells
title_fullStr Alterations in integrin expression modulates invasion of pancreatic cancer cells
title_full_unstemmed Alterations in integrin expression modulates invasion of pancreatic cancer cells
title_short Alterations in integrin expression modulates invasion of pancreatic cancer cells
title_sort alterations in integrin expression modulates invasion of pancreatic cancer cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2765436/
https://www.ncbi.nlm.nih.gov/pubmed/19825166
http://dx.doi.org/10.1186/1756-9966-28-140
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