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ConservedPrimers 2.0: A high-throughput pipeline for comparative genome referenced intron-flanking PCR primer design and its application in wheat SNP discovery

BACKGROUND: In some genomic applications it is necessary to design large numbers of PCR primers in exons flanking one or several introns on the basis of orthologous gene sequences in related species. The primer pairs designed by this target gene approach are called "intron-flanking primers"...

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Detalles Bibliográficos
Autores principales:	You, Frank M, Huo, Naxin, Gu, Yong Q, Lazo, Gerard R, Dvorak, Jan, Anderson, Olin D
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2009
Materias:	Software
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2765976/ https://www.ncbi.nlm.nih.gov/pubmed/19825183 http://dx.doi.org/10.1186/1471-2105-10-331

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