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Regulation of seed germination and seedling growth by an Arabidopsis phytocystatin isoform, AtCYS6
Phytocystatins are cysteine proteinase inhibitors in plants that are implicated in the endogenous regulation of protein turnover and defense mechanisms against insects and pathogens. A cDNA encoding a phytocystatin called AtCYS6 (Arabidopsis thaliana phytocystatin6) has been isolated. We show that A...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Springer-Verlag
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2766501/ https://www.ncbi.nlm.nih.gov/pubmed/19690865 http://dx.doi.org/10.1007/s00299-009-0762-7 |
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author | Hwang, Jung Eun Hong, Joon Ki Je, Ji Hyun Lee, Kyun Oh Kim, Dool Yi Lee, Sang Yeol Lim, Chae Oh |
author_facet | Hwang, Jung Eun Hong, Joon Ki Je, Ji Hyun Lee, Kyun Oh Kim, Dool Yi Lee, Sang Yeol Lim, Chae Oh |
author_sort | Hwang, Jung Eun |
collection | PubMed |
description | Phytocystatins are cysteine proteinase inhibitors in plants that are implicated in the endogenous regulation of protein turnover and defense mechanisms against insects and pathogens. A cDNA encoding a phytocystatin called AtCYS6 (Arabidopsis thaliana phytocystatin6) has been isolated. We show that AtCYS6 is highly expressed in dry seeds and seedlings and that it also accumulates in flowers. The persistence of AtCYS6 protein expression in seedlings was promoted by abscisic acid (ABA), a seed germination and post-germination inhibitory phytohormone. This finding was made in transgenic plants bearing an AtCYS6 promoter–β-glucuronidase (GUS) reporter construct, where we found that expression from the AtCYS6 promoter persisted after ABA treatment but was reduced under control conditions and by gibberellin(4+7) (GA(4+7)) treatment during the germination and post-germinative periods. In addition, constitutive over-expression of AtCYS6 retarded germination and seedling growth, whereas these were enhanced in an AtCYS6 knock-out mutant (cys6-2). Additionally, cysteine proteinase activities stored in seeds were inhibited by AtCYS6 in transgenic Arabidopsis. From these data, we propose that AtCYS6 expression is enhanced by the germination inhibitory phytohormone ABA and that it participates in the control of germination rate and seedling growth by inhibiting the activity of stored cysteine proteinases. |
format | Text |
id | pubmed-2766501 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-27665012009-10-26 Regulation of seed germination and seedling growth by an Arabidopsis phytocystatin isoform, AtCYS6 Hwang, Jung Eun Hong, Joon Ki Je, Ji Hyun Lee, Kyun Oh Kim, Dool Yi Lee, Sang Yeol Lim, Chae Oh Plant Cell Rep Original Paper Phytocystatins are cysteine proteinase inhibitors in plants that are implicated in the endogenous regulation of protein turnover and defense mechanisms against insects and pathogens. A cDNA encoding a phytocystatin called AtCYS6 (Arabidopsis thaliana phytocystatin6) has been isolated. We show that AtCYS6 is highly expressed in dry seeds and seedlings and that it also accumulates in flowers. The persistence of AtCYS6 protein expression in seedlings was promoted by abscisic acid (ABA), a seed germination and post-germination inhibitory phytohormone. This finding was made in transgenic plants bearing an AtCYS6 promoter–β-glucuronidase (GUS) reporter construct, where we found that expression from the AtCYS6 promoter persisted after ABA treatment but was reduced under control conditions and by gibberellin(4+7) (GA(4+7)) treatment during the germination and post-germinative periods. In addition, constitutive over-expression of AtCYS6 retarded germination and seedling growth, whereas these were enhanced in an AtCYS6 knock-out mutant (cys6-2). Additionally, cysteine proteinase activities stored in seeds were inhibited by AtCYS6 in transgenic Arabidopsis. From these data, we propose that AtCYS6 expression is enhanced by the germination inhibitory phytohormone ABA and that it participates in the control of germination rate and seedling growth by inhibiting the activity of stored cysteine proteinases. Springer-Verlag 2009-08-19 2009 /pmc/articles/PMC2766501/ /pubmed/19690865 http://dx.doi.org/10.1007/s00299-009-0762-7 Text en © The Author(s) 2009 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Original Paper Hwang, Jung Eun Hong, Joon Ki Je, Ji Hyun Lee, Kyun Oh Kim, Dool Yi Lee, Sang Yeol Lim, Chae Oh Regulation of seed germination and seedling growth by an Arabidopsis phytocystatin isoform, AtCYS6 |
title | Regulation of seed germination and seedling growth by an Arabidopsis phytocystatin isoform, AtCYS6 |
title_full | Regulation of seed germination and seedling growth by an Arabidopsis phytocystatin isoform, AtCYS6 |
title_fullStr | Regulation of seed germination and seedling growth by an Arabidopsis phytocystatin isoform, AtCYS6 |
title_full_unstemmed | Regulation of seed germination and seedling growth by an Arabidopsis phytocystatin isoform, AtCYS6 |
title_short | Regulation of seed germination and seedling growth by an Arabidopsis phytocystatin isoform, AtCYS6 |
title_sort | regulation of seed germination and seedling growth by an arabidopsis phytocystatin isoform, atcys6 |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2766501/ https://www.ncbi.nlm.nih.gov/pubmed/19690865 http://dx.doi.org/10.1007/s00299-009-0762-7 |
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