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Immunochemical studies of the lipopolysaccharides of Hafnia alvei PCM 1219 and other strains with the O-antigens containing D-glucose 1-phosphate and 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose

INTRODUCTION: Hafnia alveiis the only species of the genus Hafnia, which belongs to the family of Enterobacteriaceae. These Gram-negative bacteria are commonly distributed in the natural environment and are often the cause of human opportunistic infections. Their lipopolysaccharides (LPSs) are impor...

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Autores principales: Katzenellenbogen, Ewa, Kocharova, Nina A., Korzeniowska-Kowal, Agnieszka, Gamian, Andrzej, Bogulska, Maria, Szostko, Bernadeta, Shashkov, Alexander S., Knirel, Yuriy A.
Formato: Texto
Lenguaje:English
Publicado: Birkhäuser-Verlag 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2768797/
https://www.ncbi.nlm.nih.gov/pubmed/18836891
http://dx.doi.org/10.1007/s00005-008-0034-1
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author Katzenellenbogen, Ewa
Kocharova, Nina A.
Korzeniowska-Kowal, Agnieszka
Gamian, Andrzej
Bogulska, Maria
Szostko, Bernadeta
Shashkov, Alexander S.
Knirel, Yuriy A.
author_facet Katzenellenbogen, Ewa
Kocharova, Nina A.
Korzeniowska-Kowal, Agnieszka
Gamian, Andrzej
Bogulska, Maria
Szostko, Bernadeta
Shashkov, Alexander S.
Knirel, Yuriy A.
author_sort Katzenellenbogen, Ewa
collection PubMed
description INTRODUCTION: Hafnia alveiis the only species of the genus Hafnia, which belongs to the family of Enterobacteriaceae. These Gram-negative bacteria are commonly distributed in the natural environment and are often the cause of human opportunistic infections. Their lipopolysaccharides (LPSs) are important surface antigens which are responsible for the serological specificity and numerous cross-reactions with other enterobacterial genera. So far, 29 different O-polysaccharide (OPS, O-antigen) structures in Hafnias LPSs have been established and for some of them the molecular basis of the serological activity has been elucidated. MATERIALS AND METHODS: OPS from H. alvei strain PCM 1219 was obtained by mild acid hydrolysis of the LPS followed by gel permeation chromatography of carbohydrate material on Sephadex G-50 column. The polysaccharide structure was determined using chemical methods as well as (13)C NMR and (1)H NMR spectroscopy. For serological studies, SDS-PAGE, immunoblotting, and passive hemagglutination tests were used. RESULTS: The serological studies revealed a cross-reactivity of the LPSs of H. alvei PCM 1219 and a group of H. alvei strains with an O-antigen containing D-glucose 1-phosphate and [(R)-3-hydroxybutyramido]-D-glucose. The following structure of the OPS was established: [Image: see text] where Acyl stands for (R)-3-hydroxybutyryl and the degree of O-acetylation is ~70%. The structure of the core oligosaccharide was found to be typical of the genus Hafnia. CONCLUSIONS: Based on the OPS structure and serological results it was concluded that H. alvei strain PCM 1219 should be classified in the same serogroup as the H. alvei type strain ATCC 13337 and five other strains containing D-glucose 1-phosphate and 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose in their O-antigens.
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spelling pubmed-27687972009-10-28 Immunochemical studies of the lipopolysaccharides of Hafnia alvei PCM 1219 and other strains with the O-antigens containing D-glucose 1-phosphate and 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose Katzenellenbogen, Ewa Kocharova, Nina A. Korzeniowska-Kowal, Agnieszka Gamian, Andrzej Bogulska, Maria Szostko, Bernadeta Shashkov, Alexander S. Knirel, Yuriy A. Arch Immunol Ther Exp (Warsz) Original Article INTRODUCTION: Hafnia alveiis the only species of the genus Hafnia, which belongs to the family of Enterobacteriaceae. These Gram-negative bacteria are commonly distributed in the natural environment and are often the cause of human opportunistic infections. Their lipopolysaccharides (LPSs) are important surface antigens which are responsible for the serological specificity and numerous cross-reactions with other enterobacterial genera. So far, 29 different O-polysaccharide (OPS, O-antigen) structures in Hafnias LPSs have been established and for some of them the molecular basis of the serological activity has been elucidated. MATERIALS AND METHODS: OPS from H. alvei strain PCM 1219 was obtained by mild acid hydrolysis of the LPS followed by gel permeation chromatography of carbohydrate material on Sephadex G-50 column. The polysaccharide structure was determined using chemical methods as well as (13)C NMR and (1)H NMR spectroscopy. For serological studies, SDS-PAGE, immunoblotting, and passive hemagglutination tests were used. RESULTS: The serological studies revealed a cross-reactivity of the LPSs of H. alvei PCM 1219 and a group of H. alvei strains with an O-antigen containing D-glucose 1-phosphate and [(R)-3-hydroxybutyramido]-D-glucose. The following structure of the OPS was established: [Image: see text] where Acyl stands for (R)-3-hydroxybutyryl and the degree of O-acetylation is ~70%. The structure of the core oligosaccharide was found to be typical of the genus Hafnia. CONCLUSIONS: Based on the OPS structure and serological results it was concluded that H. alvei strain PCM 1219 should be classified in the same serogroup as the H. alvei type strain ATCC 13337 and five other strains containing D-glucose 1-phosphate and 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose in their O-antigens. Birkhäuser-Verlag 2008-10-06 2008-10 /pmc/articles/PMC2768797/ /pubmed/18836891 http://dx.doi.org/10.1007/s00005-008-0034-1 Text en © Birkhaueser 2008
spellingShingle Original Article
Katzenellenbogen, Ewa
Kocharova, Nina A.
Korzeniowska-Kowal, Agnieszka
Gamian, Andrzej
Bogulska, Maria
Szostko, Bernadeta
Shashkov, Alexander S.
Knirel, Yuriy A.
Immunochemical studies of the lipopolysaccharides of Hafnia alvei PCM 1219 and other strains with the O-antigens containing D-glucose 1-phosphate and 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose
title Immunochemical studies of the lipopolysaccharides of Hafnia alvei PCM 1219 and other strains with the O-antigens containing D-glucose 1-phosphate and 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose
title_full Immunochemical studies of the lipopolysaccharides of Hafnia alvei PCM 1219 and other strains with the O-antigens containing D-glucose 1-phosphate and 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose
title_fullStr Immunochemical studies of the lipopolysaccharides of Hafnia alvei PCM 1219 and other strains with the O-antigens containing D-glucose 1-phosphate and 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose
title_full_unstemmed Immunochemical studies of the lipopolysaccharides of Hafnia alvei PCM 1219 and other strains with the O-antigens containing D-glucose 1-phosphate and 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose
title_short Immunochemical studies of the lipopolysaccharides of Hafnia alvei PCM 1219 and other strains with the O-antigens containing D-glucose 1-phosphate and 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose
title_sort immunochemical studies of the lipopolysaccharides of hafnia alvei pcm 1219 and other strains with the o-antigens containing d-glucose 1-phosphate and 2-deoxy-2-[(r)-3-hydroxybutyramido]-d-glucose
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2768797/
https://www.ncbi.nlm.nih.gov/pubmed/18836891
http://dx.doi.org/10.1007/s00005-008-0034-1
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