Cargando…
Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors
In Saccharomyces cerevisiae, peroxisomal inheritance from mother cell to bud is conducted by the class V myosin motor, Myo2p. However, homologues of S. cerevisiae Myo2p peroxisomal receptor, Inp2p, are not readily identifiable outside the Saccharomycetaceae family. Here, we demonstrate an unexpected...
Autores principales: | , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2768826/ https://www.ncbi.nlm.nih.gov/pubmed/19822674 http://dx.doi.org/10.1083/jcb.200902117 |
_version_ | 1782173516177604608 |
---|---|
author | Chang, Jinlan Mast, Fred D. Fagarasanu, Andrei Rachubinski, Dorian A. Eitzen, Gary A. Dacks, Joel B. Rachubinski, Richard A. |
author_facet | Chang, Jinlan Mast, Fred D. Fagarasanu, Andrei Rachubinski, Dorian A. Eitzen, Gary A. Dacks, Joel B. Rachubinski, Richard A. |
author_sort | Chang, Jinlan |
collection | PubMed |
description | In Saccharomyces cerevisiae, peroxisomal inheritance from mother cell to bud is conducted by the class V myosin motor, Myo2p. However, homologues of S. cerevisiae Myo2p peroxisomal receptor, Inp2p, are not readily identifiable outside the Saccharomycetaceae family. Here, we demonstrate an unexpected role for Pex3 proteins in peroxisome inheritance. Both Pex3p and Pex3Bp are peroxisomal integral membrane proteins that function as peroxisomal receptors for class V myosin through direct interaction with the myosin globular tail. In cells lacking Pex3Bp, peroxisomes are preferentially retained by the mother cell, whereas most peroxisomes gather and are transferred en masse to the bud in cells overexpressing Pex3Bp or Pex3p. Our results reveal an unprecedented role for members of the Pex3 protein family in peroxisome motility and inheritance in addition to their well-established role in peroxisome biogenesis at the endoplasmic reticulum. Our results point to a temporal link between peroxisome formation and inheritance and delineate a general mechanism of peroxisome inheritance in eukaryotic cells. |
format | Text |
id | pubmed-2768826 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-27688262010-04-19 Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors Chang, Jinlan Mast, Fred D. Fagarasanu, Andrei Rachubinski, Dorian A. Eitzen, Gary A. Dacks, Joel B. Rachubinski, Richard A. J Cell Biol Research Articles In Saccharomyces cerevisiae, peroxisomal inheritance from mother cell to bud is conducted by the class V myosin motor, Myo2p. However, homologues of S. cerevisiae Myo2p peroxisomal receptor, Inp2p, are not readily identifiable outside the Saccharomycetaceae family. Here, we demonstrate an unexpected role for Pex3 proteins in peroxisome inheritance. Both Pex3p and Pex3Bp are peroxisomal integral membrane proteins that function as peroxisomal receptors for class V myosin through direct interaction with the myosin globular tail. In cells lacking Pex3Bp, peroxisomes are preferentially retained by the mother cell, whereas most peroxisomes gather and are transferred en masse to the bud in cells overexpressing Pex3Bp or Pex3p. Our results reveal an unprecedented role for members of the Pex3 protein family in peroxisome motility and inheritance in addition to their well-established role in peroxisome biogenesis at the endoplasmic reticulum. Our results point to a temporal link between peroxisome formation and inheritance and delineate a general mechanism of peroxisome inheritance in eukaryotic cells. The Rockefeller University Press 2009-10-19 /pmc/articles/PMC2768826/ /pubmed/19822674 http://dx.doi.org/10.1083/jcb.200902117 Text en © 2009 Chang et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Chang, Jinlan Mast, Fred D. Fagarasanu, Andrei Rachubinski, Dorian A. Eitzen, Gary A. Dacks, Joel B. Rachubinski, Richard A. Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors |
title | Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors |
title_full | Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors |
title_fullStr | Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors |
title_full_unstemmed | Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors |
title_short | Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors |
title_sort | pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class v myosin receptors |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2768826/ https://www.ncbi.nlm.nih.gov/pubmed/19822674 http://dx.doi.org/10.1083/jcb.200902117 |
work_keys_str_mv | AT changjinlan pex3peroxisomebiogenesisproteinsfunctioninperoxisomeinheritanceasclassvmyosinreceptors AT mastfredd pex3peroxisomebiogenesisproteinsfunctioninperoxisomeinheritanceasclassvmyosinreceptors AT fagarasanuandrei pex3peroxisomebiogenesisproteinsfunctioninperoxisomeinheritanceasclassvmyosinreceptors AT rachubinskidoriana pex3peroxisomebiogenesisproteinsfunctioninperoxisomeinheritanceasclassvmyosinreceptors AT eitzengarya pex3peroxisomebiogenesisproteinsfunctioninperoxisomeinheritanceasclassvmyosinreceptors AT dacksjoelb pex3peroxisomebiogenesisproteinsfunctioninperoxisomeinheritanceasclassvmyosinreceptors AT rachubinskiricharda pex3peroxisomebiogenesisproteinsfunctioninperoxisomeinheritanceasclassvmyosinreceptors |