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Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors

In Saccharomyces cerevisiae, peroxisomal inheritance from mother cell to bud is conducted by the class V myosin motor, Myo2p. However, homologues of S. cerevisiae Myo2p peroxisomal receptor, Inp2p, are not readily identifiable outside the Saccharomycetaceae family. Here, we demonstrate an unexpected...

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Autores principales: Chang, Jinlan, Mast, Fred D., Fagarasanu, Andrei, Rachubinski, Dorian A., Eitzen, Gary A., Dacks, Joel B., Rachubinski, Richard A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2768826/
https://www.ncbi.nlm.nih.gov/pubmed/19822674
http://dx.doi.org/10.1083/jcb.200902117
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author Chang, Jinlan
Mast, Fred D.
Fagarasanu, Andrei
Rachubinski, Dorian A.
Eitzen, Gary A.
Dacks, Joel B.
Rachubinski, Richard A.
author_facet Chang, Jinlan
Mast, Fred D.
Fagarasanu, Andrei
Rachubinski, Dorian A.
Eitzen, Gary A.
Dacks, Joel B.
Rachubinski, Richard A.
author_sort Chang, Jinlan
collection PubMed
description In Saccharomyces cerevisiae, peroxisomal inheritance from mother cell to bud is conducted by the class V myosin motor, Myo2p. However, homologues of S. cerevisiae Myo2p peroxisomal receptor, Inp2p, are not readily identifiable outside the Saccharomycetaceae family. Here, we demonstrate an unexpected role for Pex3 proteins in peroxisome inheritance. Both Pex3p and Pex3Bp are peroxisomal integral membrane proteins that function as peroxisomal receptors for class V myosin through direct interaction with the myosin globular tail. In cells lacking Pex3Bp, peroxisomes are preferentially retained by the mother cell, whereas most peroxisomes gather and are transferred en masse to the bud in cells overexpressing Pex3Bp or Pex3p. Our results reveal an unprecedented role for members of the Pex3 protein family in peroxisome motility and inheritance in addition to their well-established role in peroxisome biogenesis at the endoplasmic reticulum. Our results point to a temporal link between peroxisome formation and inheritance and delineate a general mechanism of peroxisome inheritance in eukaryotic cells.
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spelling pubmed-27688262010-04-19 Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors Chang, Jinlan Mast, Fred D. Fagarasanu, Andrei Rachubinski, Dorian A. Eitzen, Gary A. Dacks, Joel B. Rachubinski, Richard A. J Cell Biol Research Articles In Saccharomyces cerevisiae, peroxisomal inheritance from mother cell to bud is conducted by the class V myosin motor, Myo2p. However, homologues of S. cerevisiae Myo2p peroxisomal receptor, Inp2p, are not readily identifiable outside the Saccharomycetaceae family. Here, we demonstrate an unexpected role for Pex3 proteins in peroxisome inheritance. Both Pex3p and Pex3Bp are peroxisomal integral membrane proteins that function as peroxisomal receptors for class V myosin through direct interaction with the myosin globular tail. In cells lacking Pex3Bp, peroxisomes are preferentially retained by the mother cell, whereas most peroxisomes gather and are transferred en masse to the bud in cells overexpressing Pex3Bp or Pex3p. Our results reveal an unprecedented role for members of the Pex3 protein family in peroxisome motility and inheritance in addition to their well-established role in peroxisome biogenesis at the endoplasmic reticulum. Our results point to a temporal link between peroxisome formation and inheritance and delineate a general mechanism of peroxisome inheritance in eukaryotic cells. The Rockefeller University Press 2009-10-19 /pmc/articles/PMC2768826/ /pubmed/19822674 http://dx.doi.org/10.1083/jcb.200902117 Text en © 2009 Chang et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Chang, Jinlan
Mast, Fred D.
Fagarasanu, Andrei
Rachubinski, Dorian A.
Eitzen, Gary A.
Dacks, Joel B.
Rachubinski, Richard A.
Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors
title Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors
title_full Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors
title_fullStr Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors
title_full_unstemmed Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors
title_short Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors
title_sort pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class v myosin receptors
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2768826/
https://www.ncbi.nlm.nih.gov/pubmed/19822674
http://dx.doi.org/10.1083/jcb.200902117
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