Cargando…
Oxidative stress-induced cell cycle blockage and a protease-independent programmed cell death in microaerophilic Giardia lamblia
Giardia lamblia is a microaerophilic human gastrointestinal parasite and considered as an early-diverged eukaryote. In vitro oxidative stress generation plays a significant role in cell cycle progression and cell death of this parasite. In the present study hydrogen peroxide, metronidazole, and a mo...
Autores principales: | , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2769235/ https://www.ncbi.nlm.nih.gov/pubmed/19920926 |
_version_ | 1782173554889981952 |
---|---|
author | Ghosh, Esha Ghosh, Arjun Ghosh, Amar Nath Nozaki, Tomoyoshi Ganguly, Sandipan |
author_facet | Ghosh, Esha Ghosh, Arjun Ghosh, Amar Nath Nozaki, Tomoyoshi Ganguly, Sandipan |
author_sort | Ghosh, Esha |
collection | PubMed |
description | Giardia lamblia is a microaerophilic human gastrointestinal parasite and considered as an early-diverged eukaryote. In vitro oxidative stress generation plays a significant role in cell cycle progression and cell death of this parasite. In the present study hydrogen peroxide, metronidazole, and a modified growth medium without cysteine and ascorbic acid have been chosen as oxidative stress-inducing agents. Cell cycle progression has been found to be regulated by different types of oxidative stresses. Apoptosis is not an established pathway in Giardia, which is devoid of ideal mitochondria, but in the present investigation, apoptosis-like programmed cell death has been found by the experiments like AnnexinV-FITC assay, DNA fragmentation pattern, etc. On the contrary, Caspase-9 assay, which confirms the caspase-mediated apoptotic pathway, has been found to be negative in all the stress conditions. Protease inhibitor assay confirmed that, even in absence of any proteases, programmed cell death does occur in this primitive eukaryote. All these results signify a novel pathway of programmed suicidal death in Giardia lamblia under oxidative stress. This is the first demonstration of protease-independent programmed cell death regulation in Giardia exclusive for its own specialties. |
format | Text |
id | pubmed-2769235 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-27692352009-11-17 Oxidative stress-induced cell cycle blockage and a protease-independent programmed cell death in microaerophilic Giardia lamblia Ghosh, Esha Ghosh, Arjun Ghosh, Amar Nath Nozaki, Tomoyoshi Ganguly, Sandipan Drug Des Devel Ther Rapid Communication Giardia lamblia is a microaerophilic human gastrointestinal parasite and considered as an early-diverged eukaryote. In vitro oxidative stress generation plays a significant role in cell cycle progression and cell death of this parasite. In the present study hydrogen peroxide, metronidazole, and a modified growth medium without cysteine and ascorbic acid have been chosen as oxidative stress-inducing agents. Cell cycle progression has been found to be regulated by different types of oxidative stresses. Apoptosis is not an established pathway in Giardia, which is devoid of ideal mitochondria, but in the present investigation, apoptosis-like programmed cell death has been found by the experiments like AnnexinV-FITC assay, DNA fragmentation pattern, etc. On the contrary, Caspase-9 assay, which confirms the caspase-mediated apoptotic pathway, has been found to be negative in all the stress conditions. Protease inhibitor assay confirmed that, even in absence of any proteases, programmed cell death does occur in this primitive eukaryote. All these results signify a novel pathway of programmed suicidal death in Giardia lamblia under oxidative stress. This is the first demonstration of protease-independent programmed cell death regulation in Giardia exclusive for its own specialties. Dove Medical Press 2009-09-21 /pmc/articles/PMC2769235/ /pubmed/19920926 Text en © 2009 Ghosh et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Rapid Communication Ghosh, Esha Ghosh, Arjun Ghosh, Amar Nath Nozaki, Tomoyoshi Ganguly, Sandipan Oxidative stress-induced cell cycle blockage and a protease-independent programmed cell death in microaerophilic Giardia lamblia |
title | Oxidative stress-induced cell cycle blockage and a protease-independent programmed cell death in microaerophilic Giardia lamblia |
title_full | Oxidative stress-induced cell cycle blockage and a protease-independent programmed cell death in microaerophilic Giardia lamblia |
title_fullStr | Oxidative stress-induced cell cycle blockage and a protease-independent programmed cell death in microaerophilic Giardia lamblia |
title_full_unstemmed | Oxidative stress-induced cell cycle blockage and a protease-independent programmed cell death in microaerophilic Giardia lamblia |
title_short | Oxidative stress-induced cell cycle blockage and a protease-independent programmed cell death in microaerophilic Giardia lamblia |
title_sort | oxidative stress-induced cell cycle blockage and a protease-independent programmed cell death in microaerophilic giardia lamblia |
topic | Rapid Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2769235/ https://www.ncbi.nlm.nih.gov/pubmed/19920926 |
work_keys_str_mv | AT ghoshesha oxidativestressinducedcellcycleblockageandaproteaseindependentprogrammedcelldeathinmicroaerophilicgiardialamblia AT ghosharjun oxidativestressinducedcellcycleblockageandaproteaseindependentprogrammedcelldeathinmicroaerophilicgiardialamblia AT ghoshamarnath oxidativestressinducedcellcycleblockageandaproteaseindependentprogrammedcelldeathinmicroaerophilicgiardialamblia AT nozakitomoyoshi oxidativestressinducedcellcycleblockageandaproteaseindependentprogrammedcelldeathinmicroaerophilicgiardialamblia AT gangulysandipan oxidativestressinducedcellcycleblockageandaproteaseindependentprogrammedcelldeathinmicroaerophilicgiardialamblia |