Cargando…

Identification and characterization of merozoite surface protein 1 epitope

Malaria is an important tropical infection which urgently requires intervention of an effective vaccine. Antigenic variations of the parasite and allelic diversity of the host are main problems in the development of an effective malaria vaccine. Cytotoxic T lymphocytes (CTL) directed against Plasmod...

Descripción completa

Detalles Bibliográficos
Autores principales: Singh, Satarudra Prakash, Mishra, Bhartendu Nath
Formato: Texto
Lenguaje:English
Publicado: Biomedical Informatics 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2770364/
https://www.ncbi.nlm.nih.gov/pubmed/20011145
_version_ 1782173643201052672
author Singh, Satarudra Prakash
Mishra, Bhartendu Nath
author_facet Singh, Satarudra Prakash
Mishra, Bhartendu Nath
author_sort Singh, Satarudra Prakash
collection PubMed
description Malaria is an important tropical infection which urgently requires intervention of an effective vaccine. Antigenic variations of the parasite and allelic diversity of the host are main problems in the development of an effective malaria vaccine. Cytotoxic T lymphocytes (CTL) directed against Plasmodium falciparum­derived antigens are shown to play an important role for the protection against malaria. The merozoite surface protein 1 (MSP1) is expressed in all the four life-cycle stages of Plasmodium falciparum and did not find any sequence similarity to human and mouse reference proteins. MSP1 is a known target of the immune response and a single CTL epitope binding to the HLA­A*0201 is available for merozoite form. Here, we report the results from the computational characterization of MSP1, precursor (1720 residue) and screening of highest scoring potential CTL epitopes for 1712 overlapping peptides binding to thirty four HLA class­I alleles and twelve HLA class­I supertypes (5 HLA­A and 7 HLA­B) using bioinformatics tools. Supertypes are the clustered groups of HLA class­I molecules, representing a sets of molecules that share largely overlapping peptide binding specificity. The prediction results for MSP1 as adhesin and adhesin-like in terms of probability is 1.0. Results also show that MSP1 has orthologs to other related species as well as having non allergenicity and single transmembrane properties demonstrating its suitability as a vaccine candidate. The predicted peptides are expected to be useful in the design of multi-epitope vaccines without compromising the human population coverage.
format Text
id pubmed-2770364
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Biomedical Informatics
record_format MEDLINE/PubMed
spelling pubmed-27703642009-12-15 Identification and characterization of merozoite surface protein 1 epitope Singh, Satarudra Prakash Mishra, Bhartendu Nath Bioinformation Hypothesis Malaria is an important tropical infection which urgently requires intervention of an effective vaccine. Antigenic variations of the parasite and allelic diversity of the host are main problems in the development of an effective malaria vaccine. Cytotoxic T lymphocytes (CTL) directed against Plasmodium falciparum­derived antigens are shown to play an important role for the protection against malaria. The merozoite surface protein 1 (MSP1) is expressed in all the four life-cycle stages of Plasmodium falciparum and did not find any sequence similarity to human and mouse reference proteins. MSP1 is a known target of the immune response and a single CTL epitope binding to the HLA­A*0201 is available for merozoite form. Here, we report the results from the computational characterization of MSP1, precursor (1720 residue) and screening of highest scoring potential CTL epitopes for 1712 overlapping peptides binding to thirty four HLA class­I alleles and twelve HLA class­I supertypes (5 HLA­A and 7 HLA­B) using bioinformatics tools. Supertypes are the clustered groups of HLA class­I molecules, representing a sets of molecules that share largely overlapping peptide binding specificity. The prediction results for MSP1 as adhesin and adhesin-like in terms of probability is 1.0. Results also show that MSP1 has orthologs to other related species as well as having non allergenicity and single transmembrane properties demonstrating its suitability as a vaccine candidate. The predicted peptides are expected to be useful in the design of multi-epitope vaccines without compromising the human population coverage. Biomedical Informatics 2009-08-17 /pmc/articles/PMC2770364/ /pubmed/20011145 Text en © 2009 Biomedical Informatics This is an open-access article, which permits unrestricted use, distribution, and reproduction in any medium, for non-commercial purposes, provided the original author and source are credited.
spellingShingle Hypothesis
Singh, Satarudra Prakash
Mishra, Bhartendu Nath
Identification and characterization of merozoite surface protein 1 epitope
title Identification and characterization of merozoite surface protein 1 epitope
title_full Identification and characterization of merozoite surface protein 1 epitope
title_fullStr Identification and characterization of merozoite surface protein 1 epitope
title_full_unstemmed Identification and characterization of merozoite surface protein 1 epitope
title_short Identification and characterization of merozoite surface protein 1 epitope
title_sort identification and characterization of merozoite surface protein 1 epitope
topic Hypothesis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2770364/
https://www.ncbi.nlm.nih.gov/pubmed/20011145
work_keys_str_mv AT singhsatarudraprakash identificationandcharacterizationofmerozoitesurfaceprotein1epitope
AT mishrabhartendunath identificationandcharacterizationofmerozoitesurfaceprotein1epitope