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Maternal high fat diet during pregnancy and lactation alters hepatic expression of insulin like growth factor-2 and key microRNAs in the adult offspring

BACKGROUND: miRNAs play important roles in the regulation of gene functions. Maternal dietary modifications during pregnancy and gestation have long-term effects on the offspring, but it is not known whether a maternal high fat (HF) diet during pregnancy and lactation alters expression of key miRNAs...

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Autores principales: Zhang, Junlong, Zhang, Fang, Didelot, Xavier, Bruce, Kimberley D, Cagampang, Felino R, Vatish, Manu, Hanson, Mark, Lehnert, Hendrik, Ceriello, Antonio, Byrne, Christopher D
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2770530/
https://www.ncbi.nlm.nih.gov/pubmed/19835573
http://dx.doi.org/10.1186/1471-2164-10-478
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author Zhang, Junlong
Zhang, Fang
Didelot, Xavier
Bruce, Kimberley D
Cagampang, Felino R
Vatish, Manu
Hanson, Mark
Lehnert, Hendrik
Ceriello, Antonio
Byrne, Christopher D
author_facet Zhang, Junlong
Zhang, Fang
Didelot, Xavier
Bruce, Kimberley D
Cagampang, Felino R
Vatish, Manu
Hanson, Mark
Lehnert, Hendrik
Ceriello, Antonio
Byrne, Christopher D
author_sort Zhang, Junlong
collection PubMed
description BACKGROUND: miRNAs play important roles in the regulation of gene functions. Maternal dietary modifications during pregnancy and gestation have long-term effects on the offspring, but it is not known whether a maternal high fat (HF) diet during pregnancy and lactation alters expression of key miRNAs in the offspring. RESULTS: We studied the effects of maternal HF diet on the adult offspring by feeding mice with either a HF or a chow diet prior to conception, during pregnancy and lactation, and all offspring were weaned onto the same chow diet until adulthood. Maternal HF fed offspring had markedly increased hepatic mRNA levels of peroxisome proliferator activated receptor-alpha (ppar-alpha) and carnitine palmitoyl transferase-1a (cpt-1a) as well as insulin like growth factor-2 (Igf2). A HF diet induced up-regulation of ppar-alpha and cpt-1a expression in the wild type but not in Igf2 knock out mice. Furthermore, hepatic expression of let-7c was also reduced in maternal HF fed offspring. Among 579 miRNAs measured with microarray, ~23 miRNA levels were reduced by ~1.5-4.9-fold. Reduced expression of miR-709 (a highly expressed miRNA), miR-122, miR-192, miR-194, miR-26a, let-7a, let7b and let-7c, miR-494 and miR-483* (reduced by ~4.9 fold) was validated by qPCR. We found that methyl-CpG binding protein 2 was the common predicted target for miR-709, miR-let7s, miR-122, miR-194 and miR-26a using our own purpose-built computer program. CONCLUSION: Maternal HF feeding during pregnancy and lactation induced co-ordinated and long-lasting changes in expression of Igf2, fat metabolic genes and several important miRNAs in the offspring.
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spelling pubmed-27705302009-10-30 Maternal high fat diet during pregnancy and lactation alters hepatic expression of insulin like growth factor-2 and key microRNAs in the adult offspring Zhang, Junlong Zhang, Fang Didelot, Xavier Bruce, Kimberley D Cagampang, Felino R Vatish, Manu Hanson, Mark Lehnert, Hendrik Ceriello, Antonio Byrne, Christopher D BMC Genomics Research Article BACKGROUND: miRNAs play important roles in the regulation of gene functions. Maternal dietary modifications during pregnancy and gestation have long-term effects on the offspring, but it is not known whether a maternal high fat (HF) diet during pregnancy and lactation alters expression of key miRNAs in the offspring. RESULTS: We studied the effects of maternal HF diet on the adult offspring by feeding mice with either a HF or a chow diet prior to conception, during pregnancy and lactation, and all offspring were weaned onto the same chow diet until adulthood. Maternal HF fed offspring had markedly increased hepatic mRNA levels of peroxisome proliferator activated receptor-alpha (ppar-alpha) and carnitine palmitoyl transferase-1a (cpt-1a) as well as insulin like growth factor-2 (Igf2). A HF diet induced up-regulation of ppar-alpha and cpt-1a expression in the wild type but not in Igf2 knock out mice. Furthermore, hepatic expression of let-7c was also reduced in maternal HF fed offspring. Among 579 miRNAs measured with microarray, ~23 miRNA levels were reduced by ~1.5-4.9-fold. Reduced expression of miR-709 (a highly expressed miRNA), miR-122, miR-192, miR-194, miR-26a, let-7a, let7b and let-7c, miR-494 and miR-483* (reduced by ~4.9 fold) was validated by qPCR. We found that methyl-CpG binding protein 2 was the common predicted target for miR-709, miR-let7s, miR-122, miR-194 and miR-26a using our own purpose-built computer program. CONCLUSION: Maternal HF feeding during pregnancy and lactation induced co-ordinated and long-lasting changes in expression of Igf2, fat metabolic genes and several important miRNAs in the offspring. BioMed Central 2009-10-16 /pmc/articles/PMC2770530/ /pubmed/19835573 http://dx.doi.org/10.1186/1471-2164-10-478 Text en Copyright © 2009 Zhang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Junlong
Zhang, Fang
Didelot, Xavier
Bruce, Kimberley D
Cagampang, Felino R
Vatish, Manu
Hanson, Mark
Lehnert, Hendrik
Ceriello, Antonio
Byrne, Christopher D
Maternal high fat diet during pregnancy and lactation alters hepatic expression of insulin like growth factor-2 and key microRNAs in the adult offspring
title Maternal high fat diet during pregnancy and lactation alters hepatic expression of insulin like growth factor-2 and key microRNAs in the adult offspring
title_full Maternal high fat diet during pregnancy and lactation alters hepatic expression of insulin like growth factor-2 and key microRNAs in the adult offspring
title_fullStr Maternal high fat diet during pregnancy and lactation alters hepatic expression of insulin like growth factor-2 and key microRNAs in the adult offspring
title_full_unstemmed Maternal high fat diet during pregnancy and lactation alters hepatic expression of insulin like growth factor-2 and key microRNAs in the adult offspring
title_short Maternal high fat diet during pregnancy and lactation alters hepatic expression of insulin like growth factor-2 and key microRNAs in the adult offspring
title_sort maternal high fat diet during pregnancy and lactation alters hepatic expression of insulin like growth factor-2 and key micrornas in the adult offspring
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2770530/
https://www.ncbi.nlm.nih.gov/pubmed/19835573
http://dx.doi.org/10.1186/1471-2164-10-478
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