Characterization and comparative sequence analysis of the DNA mismatch repair MSH2 and MSH7 genes from tomato

DNA mismatch repair proteins play an essential role in maintaining genomic integrity during replication and genetic recombination. We successfully isolated a full length MSH2 and partial MSH7 cDNAs from tomato, based on sequence similarity between MutS and plant MSH homologues. Semi-quantitative RT-...

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Detalles Bibliográficos
Autores principales: Tam, Sheh May, Samipak, Sompid, Britt, Anne, Chetelat, Roger T.
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2770637/
https://www.ncbi.nlm.nih.gov/pubmed/19690966
http://dx.doi.org/10.1007/s10709-009-9398-3
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author Tam, Sheh May
Samipak, Sompid
Britt, Anne
Chetelat, Roger T.
author_facet Tam, Sheh May
Samipak, Sompid
Britt, Anne
Chetelat, Roger T.
author_sort Tam, Sheh May
collection PubMed
description DNA mismatch repair proteins play an essential role in maintaining genomic integrity during replication and genetic recombination. We successfully isolated a full length MSH2 and partial MSH7 cDNAs from tomato, based on sequence similarity between MutS and plant MSH homologues. Semi-quantitative RT-PCR reveals higher levels of mRNA expression of both genes in young leaves and floral buds. Genetic mapping placed MSH2 and MSH7 on chromosomes 6 and 7, respectively, and indicates that these genes exist as single copies in the tomato genome. Analysis of protein sequences and phylogeny of the plant MSH gene family show that these proteins are evolutionarily conserved, and follow the classical model of asymmetric protein evolution. Genetic manipulation of the expression of these MSH genes in tomato will provide a potentially useful tool for modifying genetic recombination and hybrid fertility between wide crosses. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10709-009-9398-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-27706372009-11-03 Characterization and comparative sequence analysis of the DNA mismatch repair MSH2 and MSH7 genes from tomato Tam, Sheh May Samipak, Sompid Britt, Anne Chetelat, Roger T. Genetica Article DNA mismatch repair proteins play an essential role in maintaining genomic integrity during replication and genetic recombination. We successfully isolated a full length MSH2 and partial MSH7 cDNAs from tomato, based on sequence similarity between MutS and plant MSH homologues. Semi-quantitative RT-PCR reveals higher levels of mRNA expression of both genes in young leaves and floral buds. Genetic mapping placed MSH2 and MSH7 on chromosomes 6 and 7, respectively, and indicates that these genes exist as single copies in the tomato genome. Analysis of protein sequences and phylogeny of the plant MSH gene family show that these proteins are evolutionarily conserved, and follow the classical model of asymmetric protein evolution. Genetic manipulation of the expression of these MSH genes in tomato will provide a potentially useful tool for modifying genetic recombination and hybrid fertility between wide crosses. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10709-009-9398-3) contains supplementary material, which is available to authorized users. Springer Netherlands 2009-08-19 2009 /pmc/articles/PMC2770637/ /pubmed/19690966 http://dx.doi.org/10.1007/s10709-009-9398-3 Text en © The Author(s) 2009 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Tam, Sheh May
Samipak, Sompid
Britt, Anne
Chetelat, Roger T.
Characterization and comparative sequence analysis of the DNA mismatch repair MSH2 and MSH7 genes from tomato
title Characterization and comparative sequence analysis of the DNA mismatch repair MSH2 and MSH7 genes from tomato
title_full Characterization and comparative sequence analysis of the DNA mismatch repair MSH2 and MSH7 genes from tomato
title_fullStr Characterization and comparative sequence analysis of the DNA mismatch repair MSH2 and MSH7 genes from tomato
title_full_unstemmed Characterization and comparative sequence analysis of the DNA mismatch repair MSH2 and MSH7 genes from tomato
title_short Characterization and comparative sequence analysis of the DNA mismatch repair MSH2 and MSH7 genes from tomato
title_sort characterization and comparative sequence analysis of the dna mismatch repair msh2 and msh7 genes from tomato
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2770637/
https://www.ncbi.nlm.nih.gov/pubmed/19690966
http://dx.doi.org/10.1007/s10709-009-9398-3
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