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Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells

Promoterless gene trap vectors have been widely used for high-efficiency gene targeting and random mutagenesis in embryonic stem (ES) cells. Unfortunately, such vectors are only effective for genes expressed in ES cells and this has prompted the development of expression-independent vectors. These p...

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Detalles Bibliográficos
Autores principales:	Tsakiridis, Anestis, Tzouanacou, Elena, Rahman, Afifah, Colby, Douglas, Axton, Richard, Chambers, Ian, Wilson, Valerie, Forrester, Lesley, Brickman, Joshua M.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2009
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2770648/ https://www.ncbi.nlm.nih.gov/pubmed/19692586 http://dx.doi.org/10.1093/nar/gkp640

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