Pyrosequencing of small non-coding RNAs in HIV-1 infected cells: evidence for the processing of a viral-cellular double-stranded RNA hybrid

Small non-coding RNAs of 18–25 nt in length can regulate gene expression through the RNA interference (RNAi) pathway. To characterize small RNAs in HIV-1-infected cells, we performed linker-ligated cloning followed by high-throughput pyrosequencing. Here, we report the composition of small RNAs in H...

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Autores principales: Yeung, Man Lung, Bennasser, Yamina, Watashi, Koichi, Le, Shu-Yun, Houzet, Laurent, Jeang, Kuan-Teh
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2770672/
https://www.ncbi.nlm.nih.gov/pubmed/19729508
http://dx.doi.org/10.1093/nar/gkp707
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author Yeung, Man Lung
Bennasser, Yamina
Watashi, Koichi
Le, Shu-Yun
Houzet, Laurent
Jeang, Kuan-Teh
author_facet Yeung, Man Lung
Bennasser, Yamina
Watashi, Koichi
Le, Shu-Yun
Houzet, Laurent
Jeang, Kuan-Teh
author_sort Yeung, Man Lung
collection PubMed
description Small non-coding RNAs of 18–25 nt in length can regulate gene expression through the RNA interference (RNAi) pathway. To characterize small RNAs in HIV-1-infected cells, we performed linker-ligated cloning followed by high-throughput pyrosequencing. Here, we report the composition of small RNAs in HIV-1 productively infected MT4 T-cells. We identified several HIV-1 small RNA clones and a highly abundant small 18-nt RNA that is antisense to the HIV-1 primer-binding site (PBS). This 18-nt RNA apparently originated from the dsRNA hybrid formed by the HIV-1 PBS and the 3′ end of the human cellular tRNAlys3. It was found to associate with the Ago2 protein, suggesting its possible function in the cellular RNAi machinery for targeting HIV-1.
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spelling pubmed-27706722009-10-30 Pyrosequencing of small non-coding RNAs in HIV-1 infected cells: evidence for the processing of a viral-cellular double-stranded RNA hybrid Yeung, Man Lung Bennasser, Yamina Watashi, Koichi Le, Shu-Yun Houzet, Laurent Jeang, Kuan-Teh Nucleic Acids Res RNA Small non-coding RNAs of 18–25 nt in length can regulate gene expression through the RNA interference (RNAi) pathway. To characterize small RNAs in HIV-1-infected cells, we performed linker-ligated cloning followed by high-throughput pyrosequencing. Here, we report the composition of small RNAs in HIV-1 productively infected MT4 T-cells. We identified several HIV-1 small RNA clones and a highly abundant small 18-nt RNA that is antisense to the HIV-1 primer-binding site (PBS). This 18-nt RNA apparently originated from the dsRNA hybrid formed by the HIV-1 PBS and the 3′ end of the human cellular tRNAlys3. It was found to associate with the Ago2 protein, suggesting its possible function in the cellular RNAi machinery for targeting HIV-1. Oxford University Press 2009-10 2009-09-03 /pmc/articles/PMC2770672/ /pubmed/19729508 http://dx.doi.org/10.1093/nar/gkp707 Text en © The Author(s) 2009. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA
Yeung, Man Lung
Bennasser, Yamina
Watashi, Koichi
Le, Shu-Yun
Houzet, Laurent
Jeang, Kuan-Teh
Pyrosequencing of small non-coding RNAs in HIV-1 infected cells: evidence for the processing of a viral-cellular double-stranded RNA hybrid
title Pyrosequencing of small non-coding RNAs in HIV-1 infected cells: evidence for the processing of a viral-cellular double-stranded RNA hybrid
title_full Pyrosequencing of small non-coding RNAs in HIV-1 infected cells: evidence for the processing of a viral-cellular double-stranded RNA hybrid
title_fullStr Pyrosequencing of small non-coding RNAs in HIV-1 infected cells: evidence for the processing of a viral-cellular double-stranded RNA hybrid
title_full_unstemmed Pyrosequencing of small non-coding RNAs in HIV-1 infected cells: evidence for the processing of a viral-cellular double-stranded RNA hybrid
title_short Pyrosequencing of small non-coding RNAs in HIV-1 infected cells: evidence for the processing of a viral-cellular double-stranded RNA hybrid
title_sort pyrosequencing of small non-coding rnas in hiv-1 infected cells: evidence for the processing of a viral-cellular double-stranded rna hybrid
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2770672/
https://www.ncbi.nlm.nih.gov/pubmed/19729508
http://dx.doi.org/10.1093/nar/gkp707
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