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GAP-43 expression correlates with spinal motoneuron regeneration following root avulsion
BACKGROUND: The growth-associated protein GAP-43 plays a crucial role in axonal regeneration in injured neurons. METHODS: We have used immunohistochemistry to investigate the expression of GAP-43 in spinal motoneurons during nerve reconstruction following root avulsion in the neonatal and adult rats...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2771005/ https://www.ncbi.nlm.nih.gov/pubmed/19852861 http://dx.doi.org/10.1186/1749-7221-4-18 |
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author | Yuan, Qiuju Hu, Bing Su, Huanxing So, Kwok-Fai Lin, Zhixiu Wu, Wutian |
author_facet | Yuan, Qiuju Hu, Bing Su, Huanxing So, Kwok-Fai Lin, Zhixiu Wu, Wutian |
author_sort | Yuan, Qiuju |
collection | PubMed |
description | BACKGROUND: The growth-associated protein GAP-43 plays a crucial role in axonal regeneration in injured neurons. METHODS: We have used immunohistochemistry to investigate the expression of GAP-43 in spinal motoneurons during nerve reconstruction following root avulsion in the neonatal and adult rats. RESULTS: Following the injury, GAP-43-immunoreactivity (IR) could be found in adult avulsed motoneurons as early as 1 day, increased from 3 to 7 days and reached a maximal level at 2 weeks post-injury. The up-regulation of GAP-43 in adult avulsed motoneurons was accompanied with the axonal regeneration indicated by numerous regenerating motor axons entering the reimplanted ventral root and nerve. In contrast, GAP-43-IR could not be found in the neonatal avulsed motoneurons at any examined post-injury time points. This failure of up-regulation of GAP-43 was coincident with no axonal regeneration in the reimplanted nerve in the neonatal rats. CONCLUSION: Close association of GAP-43 expression and capacity of regeneration in reimplanted spinal nerve of avulsed motoneurons suggests that GAP-43 is a potential therapeutic target for treatment of root avulsion of brachial plexus. |
format | Text |
id | pubmed-2771005 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-27710052009-10-31 GAP-43 expression correlates with spinal motoneuron regeneration following root avulsion Yuan, Qiuju Hu, Bing Su, Huanxing So, Kwok-Fai Lin, Zhixiu Wu, Wutian J Brachial Plex Peripher Nerve Inj Research Article BACKGROUND: The growth-associated protein GAP-43 plays a crucial role in axonal regeneration in injured neurons. METHODS: We have used immunohistochemistry to investigate the expression of GAP-43 in spinal motoneurons during nerve reconstruction following root avulsion in the neonatal and adult rats. RESULTS: Following the injury, GAP-43-immunoreactivity (IR) could be found in adult avulsed motoneurons as early as 1 day, increased from 3 to 7 days and reached a maximal level at 2 weeks post-injury. The up-regulation of GAP-43 in adult avulsed motoneurons was accompanied with the axonal regeneration indicated by numerous regenerating motor axons entering the reimplanted ventral root and nerve. In contrast, GAP-43-IR could not be found in the neonatal avulsed motoneurons at any examined post-injury time points. This failure of up-regulation of GAP-43 was coincident with no axonal regeneration in the reimplanted nerve in the neonatal rats. CONCLUSION: Close association of GAP-43 expression and capacity of regeneration in reimplanted spinal nerve of avulsed motoneurons suggests that GAP-43 is a potential therapeutic target for treatment of root avulsion of brachial plexus. BioMed Central 2009-10-25 /pmc/articles/PMC2771005/ /pubmed/19852861 http://dx.doi.org/10.1186/1749-7221-4-18 Text en Copyright © 2009 Yuan et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yuan, Qiuju Hu, Bing Su, Huanxing So, Kwok-Fai Lin, Zhixiu Wu, Wutian GAP-43 expression correlates with spinal motoneuron regeneration following root avulsion |
title | GAP-43 expression correlates with spinal motoneuron regeneration following root avulsion |
title_full | GAP-43 expression correlates with spinal motoneuron regeneration following root avulsion |
title_fullStr | GAP-43 expression correlates with spinal motoneuron regeneration following root avulsion |
title_full_unstemmed | GAP-43 expression correlates with spinal motoneuron regeneration following root avulsion |
title_short | GAP-43 expression correlates with spinal motoneuron regeneration following root avulsion |
title_sort | gap-43 expression correlates with spinal motoneuron regeneration following root avulsion |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2771005/ https://www.ncbi.nlm.nih.gov/pubmed/19852861 http://dx.doi.org/10.1186/1749-7221-4-18 |
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