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Prophylaxis with α-lipoic acid against lipopolysaccharide-induced brain injury in rats
INTRODUCTION: Lipopolysaccharide (LPS) stimulates the synthesis and release of reactive oxygen species that play an important role in the pathogenesis of tissue injuries. In this study the effect of early administration of the antioxidant α-lipoic acid (α-LA) on brain lipid peroxidation, brain hydro...
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Formato: | Texto |
Lenguaje: | English |
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Birkhäuser-Verlag
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2771127/ https://www.ncbi.nlm.nih.gov/pubmed/19333732 http://dx.doi.org/10.1007/s00005-009-0015-z |
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author | Gorąca, Anna Asłanowicz-Antkowiak, Katarzyna |
author_facet | Gorąca, Anna Asłanowicz-Antkowiak, Katarzyna |
author_sort | Gorąca, Anna |
collection | PubMed |
description | INTRODUCTION: Lipopolysaccharide (LPS) stimulates the synthesis and release of reactive oxygen species that play an important role in the pathogenesis of tissue injuries. In this study the effect of early administration of the antioxidant α-lipoic acid (α-LA) on brain lipid peroxidation, brain hydrogen peroxide (H(2)O(2)) concentration, and brain total sulfhydryl group (-SH group) content was evaluated in rats with endotoxic shock induced by administration of LPS (Escherichia coli 026:B6, 30 mg/kg i.v.) MATERIALS AND METHODS: Rats were treated intravenously with normal saline or α-LA (60 mg/kg) 30 min after LPS injection. After 5 h of observation, the animals were killed and their brains were isolated for the measurements. RESULTS: Injection of LPS alone resulted in the development of shock and oxidative stress, the latter indicated by a significant increase in brain concentrations of thiobarbituric acid-reacting substances (TBARS) and H(2)O(2) and a decrease in total brain -SH group content. Administration of α-LA after the LPS challenge resulted in an increase in total -SH group content and a decrease in TBARS and H(2)O(2) concentration in the brain tissue compared with the LPS group. CONCLUSION: The results indicate that α-LA treatment effectively protected the brain tissue against endotoxin-induced oxidative stress. Administration of LA could be a useful adjunct to clinical application in the management of septic shock. |
format | Text |
id | pubmed-2771127 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Birkhäuser-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-27711272009-11-06 Prophylaxis with α-lipoic acid against lipopolysaccharide-induced brain injury in rats Gorąca, Anna Asłanowicz-Antkowiak, Katarzyna Arch Immunol Ther Exp (Warsz) Original Article INTRODUCTION: Lipopolysaccharide (LPS) stimulates the synthesis and release of reactive oxygen species that play an important role in the pathogenesis of tissue injuries. In this study the effect of early administration of the antioxidant α-lipoic acid (α-LA) on brain lipid peroxidation, brain hydrogen peroxide (H(2)O(2)) concentration, and brain total sulfhydryl group (-SH group) content was evaluated in rats with endotoxic shock induced by administration of LPS (Escherichia coli 026:B6, 30 mg/kg i.v.) MATERIALS AND METHODS: Rats were treated intravenously with normal saline or α-LA (60 mg/kg) 30 min after LPS injection. After 5 h of observation, the animals were killed and their brains were isolated for the measurements. RESULTS: Injection of LPS alone resulted in the development of shock and oxidative stress, the latter indicated by a significant increase in brain concentrations of thiobarbituric acid-reacting substances (TBARS) and H(2)O(2) and a decrease in total brain -SH group content. Administration of α-LA after the LPS challenge resulted in an increase in total -SH group content and a decrease in TBARS and H(2)O(2) concentration in the brain tissue compared with the LPS group. CONCLUSION: The results indicate that α-LA treatment effectively protected the brain tissue against endotoxin-induced oxidative stress. Administration of LA could be a useful adjunct to clinical application in the management of septic shock. Birkhäuser-Verlag 2009-03-31 2009-04 /pmc/articles/PMC2771127/ /pubmed/19333732 http://dx.doi.org/10.1007/s00005-009-0015-z Text en © L. Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland 2009 |
spellingShingle | Original Article Gorąca, Anna Asłanowicz-Antkowiak, Katarzyna Prophylaxis with α-lipoic acid against lipopolysaccharide-induced brain injury in rats |
title | Prophylaxis with α-lipoic acid against lipopolysaccharide-induced brain injury in rats |
title_full | Prophylaxis with α-lipoic acid against lipopolysaccharide-induced brain injury in rats |
title_fullStr | Prophylaxis with α-lipoic acid against lipopolysaccharide-induced brain injury in rats |
title_full_unstemmed | Prophylaxis with α-lipoic acid against lipopolysaccharide-induced brain injury in rats |
title_short | Prophylaxis with α-lipoic acid against lipopolysaccharide-induced brain injury in rats |
title_sort | prophylaxis with α-lipoic acid against lipopolysaccharide-induced brain injury in rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2771127/ https://www.ncbi.nlm.nih.gov/pubmed/19333732 http://dx.doi.org/10.1007/s00005-009-0015-z |
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