CXCL13 is essential for lymph node initiation and is induced by retinoic acid and neuronal stimulation

Location of embryonic lymph node development is determined by the initial clustering of lymphoid tissue inducer cells. We demonstrate that both CXCL13 and CCL21 attracted E12.5–E14.5 lymphoid tissue inducer cells and that initial clustering exclusively depended on CXCL13. Retinoic acid induced early...

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Detalles Bibliográficos
Autores principales: van de Pavert, Serge A., Olivier, Brenda J., Goverse, Gera, Vondenhoff, Mark F., Greuter, Mascha, Beke, Patrick, Kusser, Kim, Höpken, Uta E., Lipp, Martin, Niederreither, Karen, Blomhoff, Rune, Sitnik, Kasia, Agace, William W., Randall, Troy D., de Jonge, Wouter J., Mebius, Reina E.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2771164/
https://www.ncbi.nlm.nih.gov/pubmed/19783990
http://dx.doi.org/10.1038/ni.1789
Descripción
Sumario:Location of embryonic lymph node development is determined by the initial clustering of lymphoid tissue inducer cells. We demonstrate that both CXCL13 and CCL21 attracted E12.5–E14.5 lymphoid tissue inducer cells and that initial clustering exclusively depended on CXCL13. Retinoic acid induced early CXCL13 expression in stromal organizer cells independent of lymphotoxin signaling. Notably, neurons adjacent to the lymph node anlagen expressed enzymes essential for retinoic acid synthesis. Furthermore, stimulation of parasymphathetic neural output in adults led to a retinoic acid receptor-dependent induction of CXCL13 in the gut. Therefore, our data show that initiation of lymph node development is controlled by retinoic acid-mediated expression of CXCL13 and suggest that retinoic acid may be provided by adjacent neurons.

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