Analysis of Short Tandem Repeats by Parallel DNA Threading

The majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As such, we demonstrate the feasibility of the trinucleotide threading (TnT) approach for scalable analysis of STRs. The TnT method represents a parallel amplification alternative...

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Detalles Bibliográficos
Autores principales: Zajac, Pawel, Öberg, Christine, Ahmadian, Afshin
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2773041/
https://www.ncbi.nlm.nih.gov/pubmed/19915680
http://dx.doi.org/10.1371/journal.pone.0007823
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author Zajac, Pawel
Öberg, Christine
Ahmadian, Afshin
author_facet Zajac, Pawel
Öberg, Christine
Ahmadian, Afshin
author_sort Zajac, Pawel
collection PubMed
description The majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As such, we demonstrate the feasibility of the trinucleotide threading (TnT) approach for scalable analysis of STRs. The TnT method represents a parallel amplification alternative that addresses the obstacles associated with multiplex PCR. In this study, analysis of the STR fragments was performed with capillary gel electrophoresis; however, it should be possible to combine our approach with the massive 454 sequencing platform to considerably increase the number of targeted STRs.
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spelling pubmed-27730412009-11-15 Analysis of Short Tandem Repeats by Parallel DNA Threading Zajac, Pawel Öberg, Christine Ahmadian, Afshin PLoS One Research Article The majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As such, we demonstrate the feasibility of the trinucleotide threading (TnT) approach for scalable analysis of STRs. The TnT method represents a parallel amplification alternative that addresses the obstacles associated with multiplex PCR. In this study, analysis of the STR fragments was performed with capillary gel electrophoresis; however, it should be possible to combine our approach with the massive 454 sequencing platform to considerably increase the number of targeted STRs. Public Library of Science 2009-11-13 /pmc/articles/PMC2773041/ /pubmed/19915680 http://dx.doi.org/10.1371/journal.pone.0007823 Text en Zajac et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zajac, Pawel
Öberg, Christine
Ahmadian, Afshin
Analysis of Short Tandem Repeats by Parallel DNA Threading
title Analysis of Short Tandem Repeats by Parallel DNA Threading
title_full Analysis of Short Tandem Repeats by Parallel DNA Threading
title_fullStr Analysis of Short Tandem Repeats by Parallel DNA Threading
title_full_unstemmed Analysis of Short Tandem Repeats by Parallel DNA Threading
title_short Analysis of Short Tandem Repeats by Parallel DNA Threading
title_sort analysis of short tandem repeats by parallel dna threading
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2773041/
https://www.ncbi.nlm.nih.gov/pubmed/19915680
http://dx.doi.org/10.1371/journal.pone.0007823
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