Cargando…

Localised and reversible TGFβ signalling switches breast cancer cells from cohesive to single cell motility

Here we use intravital imaging to demonstrate a reversible transition to a motile state as breast cancer cells spread. Imaging primary tumours reveals heterogeneity in cell morphology and motility. Two distinct modes of motility are observed: collective and single-celled. By monitoring the localisat...

Descripción completa

Detalles Bibliográficos
Autores principales: Giampieri, Silvia, Manning, Cerys, Hooper, Steven, Jones, Louise, Hill, Caroline S., Sahai, Erik
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2773241/
https://www.ncbi.nlm.nih.gov/pubmed/19838175
http://dx.doi.org/10.1038/ncb1973
_version_ 1782173855079464960
author Giampieri, Silvia
Manning, Cerys
Hooper, Steven
Jones, Louise
Hill, Caroline S.
Sahai, Erik
author_facet Giampieri, Silvia
Manning, Cerys
Hooper, Steven
Jones, Louise
Hill, Caroline S.
Sahai, Erik
author_sort Giampieri, Silvia
collection PubMed
description Here we use intravital imaging to demonstrate a reversible transition to a motile state as breast cancer cells spread. Imaging primary tumours reveals heterogeneity in cell morphology and motility. Two distinct modes of motility are observed: collective and single-celled. By monitoring the localisation of Smad2 and the activity of a TGFβ-dependent reporter gene during breast cancer cell dissemination we demonstrate that TGFβ signalling is transiently and locally activated in motile single cells. TGFβ1 switches cells from cohesive to single cell motility through a transcriptional programme involving Smad4, EGFR, Nedd9, M-RIP, FARP and RhoC. Blockade of TGFβ signalling prevents cells moving singly in vivo but does not inhibit cells moving collectively. Cells restricted to collective invasion are capable of lymphatic invasion but not blood-borne metastasis. Constitutive TGFβ signalling promotes single cell motility and intravasation but reduces subsequent growth in the lungs. Thus, transient TGFβ signalling is critical for blood-borne metastasis.
format Text
id pubmed-2773241
institution National Center for Biotechnology Information
language English
publishDate 2009
record_format MEDLINE/PubMed
spelling pubmed-27732412010-05-01 Localised and reversible TGFβ signalling switches breast cancer cells from cohesive to single cell motility Giampieri, Silvia Manning, Cerys Hooper, Steven Jones, Louise Hill, Caroline S. Sahai, Erik Nat Cell Biol Article Here we use intravital imaging to demonstrate a reversible transition to a motile state as breast cancer cells spread. Imaging primary tumours reveals heterogeneity in cell morphology and motility. Two distinct modes of motility are observed: collective and single-celled. By monitoring the localisation of Smad2 and the activity of a TGFβ-dependent reporter gene during breast cancer cell dissemination we demonstrate that TGFβ signalling is transiently and locally activated in motile single cells. TGFβ1 switches cells from cohesive to single cell motility through a transcriptional programme involving Smad4, EGFR, Nedd9, M-RIP, FARP and RhoC. Blockade of TGFβ signalling prevents cells moving singly in vivo but does not inhibit cells moving collectively. Cells restricted to collective invasion are capable of lymphatic invasion but not blood-borne metastasis. Constitutive TGFβ signalling promotes single cell motility and intravasation but reduces subsequent growth in the lungs. Thus, transient TGFβ signalling is critical for blood-borne metastasis. 2009-10-18 2009-11 /pmc/articles/PMC2773241/ /pubmed/19838175 http://dx.doi.org/10.1038/ncb1973 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Giampieri, Silvia
Manning, Cerys
Hooper, Steven
Jones, Louise
Hill, Caroline S.
Sahai, Erik
Localised and reversible TGFβ signalling switches breast cancer cells from cohesive to single cell motility
title Localised and reversible TGFβ signalling switches breast cancer cells from cohesive to single cell motility
title_full Localised and reversible TGFβ signalling switches breast cancer cells from cohesive to single cell motility
title_fullStr Localised and reversible TGFβ signalling switches breast cancer cells from cohesive to single cell motility
title_full_unstemmed Localised and reversible TGFβ signalling switches breast cancer cells from cohesive to single cell motility
title_short Localised and reversible TGFβ signalling switches breast cancer cells from cohesive to single cell motility
title_sort localised and reversible tgfβ signalling switches breast cancer cells from cohesive to single cell motility
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2773241/
https://www.ncbi.nlm.nih.gov/pubmed/19838175
http://dx.doi.org/10.1038/ncb1973
work_keys_str_mv AT giampierisilvia localisedandreversibletgfbsignallingswitchesbreastcancercellsfromcohesivetosinglecellmotility
AT manningcerys localisedandreversibletgfbsignallingswitchesbreastcancercellsfromcohesivetosinglecellmotility
AT hoopersteven localisedandreversibletgfbsignallingswitchesbreastcancercellsfromcohesivetosinglecellmotility
AT joneslouise localisedandreversibletgfbsignallingswitchesbreastcancercellsfromcohesivetosinglecellmotility
AT hillcarolines localisedandreversibletgfbsignallingswitchesbreastcancercellsfromcohesivetosinglecellmotility
AT sahaierik localisedandreversibletgfbsignallingswitchesbreastcancercellsfromcohesivetosinglecellmotility