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Secreted production of an elastin-like polypeptide by Pichia pastoris

Elastin-like polypeptides (ELPs) are biocompatible designer polypeptides with inverse temperature transition behavior in solution. They have a wide variety of possible applications and a potential medical importance. Currently, production of ELPs is done at lab scale in Escherichia coli shake flask...

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Detalles Bibliográficos
Autores principales: Schipperus, Roelof, Teeuwen, Rosalie L. M., Werten, Marc W. T., Eggink, Gerrit, de Wolf, Frits A.
Formato: Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2773366/
https://www.ncbi.nlm.nih.gov/pubmed/19565236
http://dx.doi.org/10.1007/s00253-009-2082-9
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author Schipperus, Roelof
Teeuwen, Rosalie L. M.
Werten, Marc W. T.
Eggink, Gerrit
de Wolf, Frits A.
author_facet Schipperus, Roelof
Teeuwen, Rosalie L. M.
Werten, Marc W. T.
Eggink, Gerrit
de Wolf, Frits A.
author_sort Schipperus, Roelof
collection PubMed
description Elastin-like polypeptides (ELPs) are biocompatible designer polypeptides with inverse temperature transition behavior in solution. They have a wide variety of possible applications and a potential medical importance. Currently, production of ELPs is done at lab scale in Escherichia coli shake flask cultures. With a view to future large scale production, we demonstrate secreted production of ELPs in methanol-induced fed-batch cultures of Pichia pastoris and purification directly from the culture medium. The production of ELPs by P. pastoris proved to be pH dependent within the experimental pH range of pH 3 to 7, as an increasing yield was found in cultures grown at higher pH. Because ELP produced at pH 7 was partly degraded, a pH optimum for production of ELP was found at pH 6 with a yield of 255 mg of purified intact ELP per liter of cell-free medium.
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spelling pubmed-27733662009-11-06 Secreted production of an elastin-like polypeptide by Pichia pastoris Schipperus, Roelof Teeuwen, Rosalie L. M. Werten, Marc W. T. Eggink, Gerrit de Wolf, Frits A. Appl Microbiol Biotechnol Biotechnological Products and Process Engineering Elastin-like polypeptides (ELPs) are biocompatible designer polypeptides with inverse temperature transition behavior in solution. They have a wide variety of possible applications and a potential medical importance. Currently, production of ELPs is done at lab scale in Escherichia coli shake flask cultures. With a view to future large scale production, we demonstrate secreted production of ELPs in methanol-induced fed-batch cultures of Pichia pastoris and purification directly from the culture medium. The production of ELPs by P. pastoris proved to be pH dependent within the experimental pH range of pH 3 to 7, as an increasing yield was found in cultures grown at higher pH. Because ELP produced at pH 7 was partly degraded, a pH optimum for production of ELP was found at pH 6 with a yield of 255 mg of purified intact ELP per liter of cell-free medium. Springer Berlin Heidelberg 2009-06-30 2009 /pmc/articles/PMC2773366/ /pubmed/19565236 http://dx.doi.org/10.1007/s00253-009-2082-9 Text en © The Author(s) 2009 Open AccessThis is an open access article distributed under the terms of the Creative Commons Attribution Noncommercial License (https://creativecommons.org/licenses/by-nc/2.0), which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Biotechnological Products and Process Engineering
Schipperus, Roelof
Teeuwen, Rosalie L. M.
Werten, Marc W. T.
Eggink, Gerrit
de Wolf, Frits A.
Secreted production of an elastin-like polypeptide by Pichia pastoris
title Secreted production of an elastin-like polypeptide by Pichia pastoris
title_full Secreted production of an elastin-like polypeptide by Pichia pastoris
title_fullStr Secreted production of an elastin-like polypeptide by Pichia pastoris
title_full_unstemmed Secreted production of an elastin-like polypeptide by Pichia pastoris
title_short Secreted production of an elastin-like polypeptide by Pichia pastoris
title_sort secreted production of an elastin-like polypeptide by pichia pastoris
topic Biotechnological Products and Process Engineering
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2773366/
https://www.ncbi.nlm.nih.gov/pubmed/19565236
http://dx.doi.org/10.1007/s00253-009-2082-9
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