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Effect of a combination of carnosine and aspirin eye drops on streptozotocin - induced diabetic cataract in rats

PURPOSE: To investigate the effect of a combination of carnosine and aspirin eye drops on the progression of diabetic cataract formation induced by streptozotocin (STZ). METHODS: Rats were made diabetic with STZ. Animals in the treated groups received carnosine, aspirin, or a combination of carnosin...

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Autores principales: Shi, Qiong, Yan, Hong, Li, Ming-Yong, Harding, John J.
Formato: Texto
Lenguaje:English
Publicado: Molecular Vision 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2773744/
https://www.ncbi.nlm.nih.gov/pubmed/19898641
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author Shi, Qiong
Yan, Hong
Li, Ming-Yong
Harding, John J.
author_facet Shi, Qiong
Yan, Hong
Li, Ming-Yong
Harding, John J.
author_sort Shi, Qiong
collection PubMed
description PURPOSE: To investigate the effect of a combination of carnosine and aspirin eye drops on the progression of diabetic cataract formation induced by streptozotocin (STZ). METHODS: Rats were made diabetic with STZ. Animals in the treated groups received carnosine, aspirin, or a combination of carnosine and aspirin as drops to the eyes. Cataract progression was monitored by slit lamp microscope and classified into four stages. At the end of 8 weeks, the animals were killed and biochemical changes were determined. Blood and urine glucose levels, body weights, food, and intake were also determined. RESULTS: About 84.4% of the rats responded to the STZ injection. There were statistically significant differences in the stage of cataract of lenses between the untreated and the treated diabetic animals and between the combination and the aspirin group at the 7th and 8th week. There was a significant decrease in the water-soluble protein in the diabetic groups compared with the control group. The three treatments improved the water-soluble protein levels, and the combination treatment had the greatest effect. The levels of thiol were remarkably decreased in the lenses of diabetic rats, except the combination group. The specific activity of glutathione peroxidase (GPx) was increased and the activities of glutathione reductase (GR) and catalase (CAT) were decreased in all the diabetic groups. CONCLUSIONS: The results indicated that carnosine, aspirin, and a combination eye drops are effective against the onset and development of diabetic cataract in rats. Most important, the effect of combination eye drops is better than aspirin only.
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spelling pubmed-27737442009-11-06 Effect of a combination of carnosine and aspirin eye drops on streptozotocin - induced diabetic cataract in rats Shi, Qiong Yan, Hong Li, Ming-Yong Harding, John J. Mol Vis Research Article PURPOSE: To investigate the effect of a combination of carnosine and aspirin eye drops on the progression of diabetic cataract formation induced by streptozotocin (STZ). METHODS: Rats were made diabetic with STZ. Animals in the treated groups received carnosine, aspirin, or a combination of carnosine and aspirin as drops to the eyes. Cataract progression was monitored by slit lamp microscope and classified into four stages. At the end of 8 weeks, the animals were killed and biochemical changes were determined. Blood and urine glucose levels, body weights, food, and intake were also determined. RESULTS: About 84.4% of the rats responded to the STZ injection. There were statistically significant differences in the stage of cataract of lenses between the untreated and the treated diabetic animals and between the combination and the aspirin group at the 7th and 8th week. There was a significant decrease in the water-soluble protein in the diabetic groups compared with the control group. The three treatments improved the water-soluble protein levels, and the combination treatment had the greatest effect. The levels of thiol were remarkably decreased in the lenses of diabetic rats, except the combination group. The specific activity of glutathione peroxidase (GPx) was increased and the activities of glutathione reductase (GR) and catalase (CAT) were decreased in all the diabetic groups. CONCLUSIONS: The results indicated that carnosine, aspirin, and a combination eye drops are effective against the onset and development of diabetic cataract in rats. Most important, the effect of combination eye drops is better than aspirin only. Molecular Vision 2009-10-21 /pmc/articles/PMC2773744/ /pubmed/19898641 Text en http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shi, Qiong
Yan, Hong
Li, Ming-Yong
Harding, John J.
Effect of a combination of carnosine and aspirin eye drops on streptozotocin - induced diabetic cataract in rats
title Effect of a combination of carnosine and aspirin eye drops on streptozotocin - induced diabetic cataract in rats
title_full Effect of a combination of carnosine and aspirin eye drops on streptozotocin - induced diabetic cataract in rats
title_fullStr Effect of a combination of carnosine and aspirin eye drops on streptozotocin - induced diabetic cataract in rats
title_full_unstemmed Effect of a combination of carnosine and aspirin eye drops on streptozotocin - induced diabetic cataract in rats
title_short Effect of a combination of carnosine and aspirin eye drops on streptozotocin - induced diabetic cataract in rats
title_sort effect of a combination of carnosine and aspirin eye drops on streptozotocin - induced diabetic cataract in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2773744/
https://www.ncbi.nlm.nih.gov/pubmed/19898641
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