Cargando…
Propagation of Asian isolates of canine distemper virus (CDV) in hamster cell lines
BACKGROUNDS: The aim of this study was to confirm the propagation of various canine distemper viruses (CDV) in hamster cell lines of HmLu and BHK, since only a little is known about the possibility of propagation of CDV in rodent cells irrespective of their epidemiological importance. METHODS: The g...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774306/ https://www.ncbi.nlm.nih.gov/pubmed/19835588 http://dx.doi.org/10.1186/1751-0147-51-38 |
_version_ | 1782173925542723584 |
---|---|
author | Sultan, Serageldeen Lan, Nguyen Thi Ueda, Toshiki Yamaguchi, Ryoji Maeda, Ken Kai, Kazushige |
author_facet | Sultan, Serageldeen Lan, Nguyen Thi Ueda, Toshiki Yamaguchi, Ryoji Maeda, Ken Kai, Kazushige |
author_sort | Sultan, Serageldeen |
collection | PubMed |
description | BACKGROUNDS: The aim of this study was to confirm the propagation of various canine distemper viruses (CDV) in hamster cell lines of HmLu and BHK, since only a little is known about the possibility of propagation of CDV in rodent cells irrespective of their epidemiological importance. METHODS: The growth of CDV in hamster cell lines was monitored by titration using Vero.dogSLAMtag (Vero-DST) cells that had been proven to be susceptible to almost all field isolates of CDV, with the preparations of cell-free and cell-associated virus from the cultures infected with recent Asian isolates of CDV (13 strains) and by observing the development of cytopathic effect (CPE) in infected cultures of hamster cell lines. RESULTS: Eleven of 13 strains grew in HmLu cells, and 12 of 13 strains grew in BHK cells with apparent CPE of cell fusion in the late stage of infection. Two strains and a strain of Asia 1 group could not grow in HmLu cells and BHK cells, respectively. CONCLUSION: The present study demonstrates at the first time that hamster cell lines can propagate the majority of Asian field isolates of CDV. The usage of two hamster cell lines suggested to be useful to characterize the field isolates biologically. |
format | Text |
id | pubmed-2774306 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-27743062009-11-07 Propagation of Asian isolates of canine distemper virus (CDV) in hamster cell lines Sultan, Serageldeen Lan, Nguyen Thi Ueda, Toshiki Yamaguchi, Ryoji Maeda, Ken Kai, Kazushige Acta Vet Scand Research BACKGROUNDS: The aim of this study was to confirm the propagation of various canine distemper viruses (CDV) in hamster cell lines of HmLu and BHK, since only a little is known about the possibility of propagation of CDV in rodent cells irrespective of their epidemiological importance. METHODS: The growth of CDV in hamster cell lines was monitored by titration using Vero.dogSLAMtag (Vero-DST) cells that had been proven to be susceptible to almost all field isolates of CDV, with the preparations of cell-free and cell-associated virus from the cultures infected with recent Asian isolates of CDV (13 strains) and by observing the development of cytopathic effect (CPE) in infected cultures of hamster cell lines. RESULTS: Eleven of 13 strains grew in HmLu cells, and 12 of 13 strains grew in BHK cells with apparent CPE of cell fusion in the late stage of infection. Two strains and a strain of Asia 1 group could not grow in HmLu cells and BHK cells, respectively. CONCLUSION: The present study demonstrates at the first time that hamster cell lines can propagate the majority of Asian field isolates of CDV. The usage of two hamster cell lines suggested to be useful to characterize the field isolates biologically. BioMed Central 2009-10-16 /pmc/articles/PMC2774306/ /pubmed/19835588 http://dx.doi.org/10.1186/1751-0147-51-38 Text en Copyright © 2009 Sultan et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Sultan, Serageldeen Lan, Nguyen Thi Ueda, Toshiki Yamaguchi, Ryoji Maeda, Ken Kai, Kazushige Propagation of Asian isolates of canine distemper virus (CDV) in hamster cell lines |
title | Propagation of Asian isolates of canine distemper virus (CDV) in hamster cell lines |
title_full | Propagation of Asian isolates of canine distemper virus (CDV) in hamster cell lines |
title_fullStr | Propagation of Asian isolates of canine distemper virus (CDV) in hamster cell lines |
title_full_unstemmed | Propagation of Asian isolates of canine distemper virus (CDV) in hamster cell lines |
title_short | Propagation of Asian isolates of canine distemper virus (CDV) in hamster cell lines |
title_sort | propagation of asian isolates of canine distemper virus (cdv) in hamster cell lines |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774306/ https://www.ncbi.nlm.nih.gov/pubmed/19835588 http://dx.doi.org/10.1186/1751-0147-51-38 |
work_keys_str_mv | AT sultanserageldeen propagationofasianisolatesofcaninedistemperviruscdvinhamstercelllines AT lannguyenthi propagationofasianisolatesofcaninedistemperviruscdvinhamstercelllines AT uedatoshiki propagationofasianisolatesofcaninedistemperviruscdvinhamstercelllines AT yamaguchiryoji propagationofasianisolatesofcaninedistemperviruscdvinhamstercelllines AT maedaken propagationofasianisolatesofcaninedistemperviruscdvinhamstercelllines AT kaikazushige propagationofasianisolatesofcaninedistemperviruscdvinhamstercelllines |