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Preliminary molecular characterization of the human pathogen Angiostrongylus cantonensis

BACKGROUND: Human angiostrongyliasis is an emerging food-borne public health problem, with the number of cases increasing worldwide, especially in mainland China. Angiostrongylus cantonensis is the causative agent of this severe disease. However, little is known about the genetics and basic biology...

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Autores principales: He, Hualiang, Cheng, Mei, Yang, Xiao, Meng, Jinxiu, He, Ai, Zheng, Xiaoying, Li, Zhuoya, Guo, Pengjuan, Pan, Zhihua, Zhan, Ximei
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774698/
https://www.ncbi.nlm.nih.gov/pubmed/19852860
http://dx.doi.org/10.1186/1471-2199-10-97
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author He, Hualiang
Cheng, Mei
Yang, Xiao
Meng, Jinxiu
He, Ai
Zheng, Xiaoying
Li, Zhuoya
Guo, Pengjuan
Pan, Zhihua
Zhan, Ximei
author_facet He, Hualiang
Cheng, Mei
Yang, Xiao
Meng, Jinxiu
He, Ai
Zheng, Xiaoying
Li, Zhuoya
Guo, Pengjuan
Pan, Zhihua
Zhan, Ximei
author_sort He, Hualiang
collection PubMed
description BACKGROUND: Human angiostrongyliasis is an emerging food-borne public health problem, with the number of cases increasing worldwide, especially in mainland China. Angiostrongylus cantonensis is the causative agent of this severe disease. However, little is known about the genetics and basic biology of A. cantonensis. RESULTS: A cDNA library of A. cantonensis fourth-stage larvae was constructed, and ~1,200 clones were sequenced. Bioinformatic analyses revealed 378 cDNA clusters, 54.2% of which matched known genes at a cutoff expectation value of 10(-20). Of these 378 unique cDNAs, 168 contained open reading frames encoding proteins containing an average of 238 amino acids. Characterization of the functions of these encoded proteins by Gene Ontology analysis showed enrichment in proteins with binding and catalytic activity. The observed pattern of enzymes involved in protein metabolism, lipid metabolism and glycolysis may reflect the central nervous system habitat of this pathogen. Four proteins were tested for their immunogenicity using enzyme-linked immunosorbent assays and histopathological examinations. The specificity of each of the four proteins was superior to that of crude somatic and excretory/secretory antigens of larvae, although their sensitivity was relatively low. We further showed that mice immunized with recombinant cystatin, a product of one of the four cDNA candidate genes, were partially protected from A. cantonensis infection. CONCLUSION: The data presented here substantially expand the available genetic information about the human pathogen A. cantonensis, and should be a significant resource for angiostrongyliasis researchers. As such, this work serves as a starting point for molecular approaches for diagnosing and controlling human angiostrongyliasis.
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spelling pubmed-27746982009-11-10 Preliminary molecular characterization of the human pathogen Angiostrongylus cantonensis He, Hualiang Cheng, Mei Yang, Xiao Meng, Jinxiu He, Ai Zheng, Xiaoying Li, Zhuoya Guo, Pengjuan Pan, Zhihua Zhan, Ximei BMC Mol Biol Research Article BACKGROUND: Human angiostrongyliasis is an emerging food-borne public health problem, with the number of cases increasing worldwide, especially in mainland China. Angiostrongylus cantonensis is the causative agent of this severe disease. However, little is known about the genetics and basic biology of A. cantonensis. RESULTS: A cDNA library of A. cantonensis fourth-stage larvae was constructed, and ~1,200 clones were sequenced. Bioinformatic analyses revealed 378 cDNA clusters, 54.2% of which matched known genes at a cutoff expectation value of 10(-20). Of these 378 unique cDNAs, 168 contained open reading frames encoding proteins containing an average of 238 amino acids. Characterization of the functions of these encoded proteins by Gene Ontology analysis showed enrichment in proteins with binding and catalytic activity. The observed pattern of enzymes involved in protein metabolism, lipid metabolism and glycolysis may reflect the central nervous system habitat of this pathogen. Four proteins were tested for their immunogenicity using enzyme-linked immunosorbent assays and histopathological examinations. The specificity of each of the four proteins was superior to that of crude somatic and excretory/secretory antigens of larvae, although their sensitivity was relatively low. We further showed that mice immunized with recombinant cystatin, a product of one of the four cDNA candidate genes, were partially protected from A. cantonensis infection. CONCLUSION: The data presented here substantially expand the available genetic information about the human pathogen A. cantonensis, and should be a significant resource for angiostrongyliasis researchers. As such, this work serves as a starting point for molecular approaches for diagnosing and controlling human angiostrongyliasis. BioMed Central 2009-10-25 /pmc/articles/PMC2774698/ /pubmed/19852860 http://dx.doi.org/10.1186/1471-2199-10-97 Text en Copyright © 2009 He et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
He, Hualiang
Cheng, Mei
Yang, Xiao
Meng, Jinxiu
He, Ai
Zheng, Xiaoying
Li, Zhuoya
Guo, Pengjuan
Pan, Zhihua
Zhan, Ximei
Preliminary molecular characterization of the human pathogen Angiostrongylus cantonensis
title Preliminary molecular characterization of the human pathogen Angiostrongylus cantonensis
title_full Preliminary molecular characterization of the human pathogen Angiostrongylus cantonensis
title_fullStr Preliminary molecular characterization of the human pathogen Angiostrongylus cantonensis
title_full_unstemmed Preliminary molecular characterization of the human pathogen Angiostrongylus cantonensis
title_short Preliminary molecular characterization of the human pathogen Angiostrongylus cantonensis
title_sort preliminary molecular characterization of the human pathogen angiostrongylus cantonensis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774698/
https://www.ncbi.nlm.nih.gov/pubmed/19852860
http://dx.doi.org/10.1186/1471-2199-10-97
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