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Comparison of Plasmodium falciparum allelic frequency distribution in different endemic settings by high-resolution genotyping

BACKGROUND: The diversity of genotyping markers of Plasmodium falciparum depends on transmission intensity. It has been reported that the diversity of the merozoite surface protein 2 (msp2) is greater in areas of high compared to low endemicity, however, results for msp1 were inconsistent. These pre...

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Autores principales: Schoepflin, Sonja, Valsangiacomo, Francesca, Lin, Enmoore, Kiniboro, Benson, Mueller, Ivo, Felger, Ingrid
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774868/
https://www.ncbi.nlm.nih.gov/pubmed/19878560
http://dx.doi.org/10.1186/1475-2875-8-250
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author Schoepflin, Sonja
Valsangiacomo, Francesca
Lin, Enmoore
Kiniboro, Benson
Mueller, Ivo
Felger, Ingrid
author_facet Schoepflin, Sonja
Valsangiacomo, Francesca
Lin, Enmoore
Kiniboro, Benson
Mueller, Ivo
Felger, Ingrid
author_sort Schoepflin, Sonja
collection PubMed
description BACKGROUND: The diversity of genotyping markers of Plasmodium falciparum depends on transmission intensity. It has been reported that the diversity of the merozoite surface protein 2 (msp2) is greater in areas of high compared to low endemicity, however, results for msp1 were inconsistent. These previous reports relied on low resolution genotyping techniques. METHODS: In the present study, a high-resolution capillary electrophoresis-based technique was applied to genotype samples from areas of different endemicity in Papua New Guinea and Tanzania. For both endemic settings, the diversity of msp1 and msp2 was investigated; the mean multiplicity of infection (MOI) and the F(ST )values were determined to investigate whether more accurate sizing generates different results. RESULTS AND CONCLUSION: The results of the present study confirmed previous reports of a higher mean MOI for both marker genes and increased genetic diversity in areas of higher endemicity as estimated by the total number of distinct alleles for msp2. For msp1 a minor increase in diversity was observed. Measures of between population variance in allele frequencies (F(ST)) indicated little genetic differentiation for both marker genes between the two populations from different endemic settings. MOI adjusted for the probability of multiple infections sharing the same allele was estimated by using the msp2 allele frequency distribution and the distribution of observed numbers of concurrent infections. For the high-resolution typing technique applied in this study, this adjustment made little difference to the estimated mean MOI compared to the observed mean MOI.
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spelling pubmed-27748682009-11-10 Comparison of Plasmodium falciparum allelic frequency distribution in different endemic settings by high-resolution genotyping Schoepflin, Sonja Valsangiacomo, Francesca Lin, Enmoore Kiniboro, Benson Mueller, Ivo Felger, Ingrid Malar J Research BACKGROUND: The diversity of genotyping markers of Plasmodium falciparum depends on transmission intensity. It has been reported that the diversity of the merozoite surface protein 2 (msp2) is greater in areas of high compared to low endemicity, however, results for msp1 were inconsistent. These previous reports relied on low resolution genotyping techniques. METHODS: In the present study, a high-resolution capillary electrophoresis-based technique was applied to genotype samples from areas of different endemicity in Papua New Guinea and Tanzania. For both endemic settings, the diversity of msp1 and msp2 was investigated; the mean multiplicity of infection (MOI) and the F(ST )values were determined to investigate whether more accurate sizing generates different results. RESULTS AND CONCLUSION: The results of the present study confirmed previous reports of a higher mean MOI for both marker genes and increased genetic diversity in areas of higher endemicity as estimated by the total number of distinct alleles for msp2. For msp1 a minor increase in diversity was observed. Measures of between population variance in allele frequencies (F(ST)) indicated little genetic differentiation for both marker genes between the two populations from different endemic settings. MOI adjusted for the probability of multiple infections sharing the same allele was estimated by using the msp2 allele frequency distribution and the distribution of observed numbers of concurrent infections. For the high-resolution typing technique applied in this study, this adjustment made little difference to the estimated mean MOI compared to the observed mean MOI. BioMed Central 2009-10-30 /pmc/articles/PMC2774868/ /pubmed/19878560 http://dx.doi.org/10.1186/1475-2875-8-250 Text en Copyright © 2009 Schoepflin et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Schoepflin, Sonja
Valsangiacomo, Francesca
Lin, Enmoore
Kiniboro, Benson
Mueller, Ivo
Felger, Ingrid
Comparison of Plasmodium falciparum allelic frequency distribution in different endemic settings by high-resolution genotyping
title Comparison of Plasmodium falciparum allelic frequency distribution in different endemic settings by high-resolution genotyping
title_full Comparison of Plasmodium falciparum allelic frequency distribution in different endemic settings by high-resolution genotyping
title_fullStr Comparison of Plasmodium falciparum allelic frequency distribution in different endemic settings by high-resolution genotyping
title_full_unstemmed Comparison of Plasmodium falciparum allelic frequency distribution in different endemic settings by high-resolution genotyping
title_short Comparison of Plasmodium falciparum allelic frequency distribution in different endemic settings by high-resolution genotyping
title_sort comparison of plasmodium falciparum allelic frequency distribution in different endemic settings by high-resolution genotyping
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774868/
https://www.ncbi.nlm.nih.gov/pubmed/19878560
http://dx.doi.org/10.1186/1475-2875-8-250
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