Cargando…
Transcriptional Regulation and Biological Functions of Selenium-Binding Protein 1 in Colorectal Cancer In Vitro and in Nude Mouse Xenografts
BACKGROUND: It has been shown that selenium-binding protein 1 (SBP1) is significantly downregulated in different human cancers. Its regulation and function have not yet been established. METHODOLOGY AND PRINCIPAL FINDINGS: We show that the SBP1 promoter is hypermethylated in colon cancer tissues and...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774949/ https://www.ncbi.nlm.nih.gov/pubmed/19924303 http://dx.doi.org/10.1371/journal.pone.0007774 |
_version_ | 1782173979646099456 |
---|---|
author | Pohl, Nicole M. Tong, Chang Fang, Wenfeng Bi, Xiuli Li, Tianhong Yang, Wancai |
author_facet | Pohl, Nicole M. Tong, Chang Fang, Wenfeng Bi, Xiuli Li, Tianhong Yang, Wancai |
author_sort | Pohl, Nicole M. |
collection | PubMed |
description | BACKGROUND: It has been shown that selenium-binding protein 1 (SBP1) is significantly downregulated in different human cancers. Its regulation and function have not yet been established. METHODOLOGY AND PRINCIPAL FINDINGS: We show that the SBP1 promoter is hypermethylated in colon cancer tissues and human colon cancer cells. Treatment with 5′-Aza-2′-deoxycytidine leads to demethylation of the SBP1 promoter and to an increase of SBP1 promoter activity, rescues SBP1 mRNA and protein expression in human colon cancer cells. Additionally, overexpression of SBP1 sensitizes colon cancer cells to H(2)O(2)-induced apoptosis, inhibits cancer cell migration in vitro and inhibits tumor growth in nude mice. CONCLUSION AND SIGNIFICANCE: These data demonstrate that SBP1 has tumor suppressor functions that are inhibited in colorectal cancer through epigenetic silencing. |
format | Text |
id | pubmed-2774949 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-27749492009-11-19 Transcriptional Regulation and Biological Functions of Selenium-Binding Protein 1 in Colorectal Cancer In Vitro and in Nude Mouse Xenografts Pohl, Nicole M. Tong, Chang Fang, Wenfeng Bi, Xiuli Li, Tianhong Yang, Wancai PLoS One Research Article BACKGROUND: It has been shown that selenium-binding protein 1 (SBP1) is significantly downregulated in different human cancers. Its regulation and function have not yet been established. METHODOLOGY AND PRINCIPAL FINDINGS: We show that the SBP1 promoter is hypermethylated in colon cancer tissues and human colon cancer cells. Treatment with 5′-Aza-2′-deoxycytidine leads to demethylation of the SBP1 promoter and to an increase of SBP1 promoter activity, rescues SBP1 mRNA and protein expression in human colon cancer cells. Additionally, overexpression of SBP1 sensitizes colon cancer cells to H(2)O(2)-induced apoptosis, inhibits cancer cell migration in vitro and inhibits tumor growth in nude mice. CONCLUSION AND SIGNIFICANCE: These data demonstrate that SBP1 has tumor suppressor functions that are inhibited in colorectal cancer through epigenetic silencing. Public Library of Science 2009-11-16 /pmc/articles/PMC2774949/ /pubmed/19924303 http://dx.doi.org/10.1371/journal.pone.0007774 Text en Pohl et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Pohl, Nicole M. Tong, Chang Fang, Wenfeng Bi, Xiuli Li, Tianhong Yang, Wancai Transcriptional Regulation and Biological Functions of Selenium-Binding Protein 1 in Colorectal Cancer In Vitro and in Nude Mouse Xenografts |
title | Transcriptional Regulation and Biological Functions of Selenium-Binding Protein 1 in Colorectal Cancer In Vitro and in Nude Mouse Xenografts |
title_full | Transcriptional Regulation and Biological Functions of Selenium-Binding Protein 1 in Colorectal Cancer In Vitro and in Nude Mouse Xenografts |
title_fullStr | Transcriptional Regulation and Biological Functions of Selenium-Binding Protein 1 in Colorectal Cancer In Vitro and in Nude Mouse Xenografts |
title_full_unstemmed | Transcriptional Regulation and Biological Functions of Selenium-Binding Protein 1 in Colorectal Cancer In Vitro and in Nude Mouse Xenografts |
title_short | Transcriptional Regulation and Biological Functions of Selenium-Binding Protein 1 in Colorectal Cancer In Vitro and in Nude Mouse Xenografts |
title_sort | transcriptional regulation and biological functions of selenium-binding protein 1 in colorectal cancer in vitro and in nude mouse xenografts |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774949/ https://www.ncbi.nlm.nih.gov/pubmed/19924303 http://dx.doi.org/10.1371/journal.pone.0007774 |
work_keys_str_mv | AT pohlnicolem transcriptionalregulationandbiologicalfunctionsofseleniumbindingprotein1incolorectalcancerinvitroandinnudemousexenografts AT tongchang transcriptionalregulationandbiologicalfunctionsofseleniumbindingprotein1incolorectalcancerinvitroandinnudemousexenografts AT fangwenfeng transcriptionalregulationandbiologicalfunctionsofseleniumbindingprotein1incolorectalcancerinvitroandinnudemousexenografts AT bixiuli transcriptionalregulationandbiologicalfunctionsofseleniumbindingprotein1incolorectalcancerinvitroandinnudemousexenografts AT litianhong transcriptionalregulationandbiologicalfunctionsofseleniumbindingprotein1incolorectalcancerinvitroandinnudemousexenografts AT yangwancai transcriptionalregulationandbiologicalfunctionsofseleniumbindingprotein1incolorectalcancerinvitroandinnudemousexenografts |