A simple, flexible and efficient PCR-fusion/Gateway cloning procedure for gene fusion, site-directed mutagenesis, short sequence insertion and domain deletions and swaps

BACKGROUND: The progress and completion of various plant genome sequencing projects has paved the way for diverse functional genomic studies that involve cloning, modification and subsequent expression of target genes. This requires flexible and efficient procedures for generating binary vectors con...

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Detalles Bibliográficos
Autores principales: Atanassov, Ivan I, Atanassov, Ilian I, Etchells, J Peter, Turner, Simon R
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2775020/
https://www.ncbi.nlm.nih.gov/pubmed/19863796
http://dx.doi.org/10.1186/1746-4811-5-14

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