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Activation of Satellite Glial Cells in Rat Trigeminal Ganglion after Upper Molar Extraction

The neurons in the trigeminal ganglion (TG) are surrounded by satellite glial cells (SGCs), which passively support the function of the neurons, but little is known about the interactions between SGCs and TG neurons after peripheral nerve injury. To examine the effect of nerve injury on SGCs, we inv...

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Autores principales: Gunjigake, Kaori K., Goto, Tetsuya, Nakao, Kayoko, Kobayashi, Shigeru, Yamaguchi, Kazunori
Formato: Texto
Lenguaje:English
Publicado: Japan Society of Histochemistry and Cytochemistry 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2775105/
https://www.ncbi.nlm.nih.gov/pubmed/19918323
http://dx.doi.org/10.1267/ahc.09017
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author Gunjigake, Kaori K.
Goto, Tetsuya
Nakao, Kayoko
Kobayashi, Shigeru
Yamaguchi, Kazunori
author_facet Gunjigake, Kaori K.
Goto, Tetsuya
Nakao, Kayoko
Kobayashi, Shigeru
Yamaguchi, Kazunori
author_sort Gunjigake, Kaori K.
collection PubMed
description The neurons in the trigeminal ganglion (TG) are surrounded by satellite glial cells (SGCs), which passively support the function of the neurons, but little is known about the interactions between SGCs and TG neurons after peripheral nerve injury. To examine the effect of nerve injury on SGCs, we investigated the activation of SGCs after neuronal damage due to the extraction of the upper molars in rats. Three, 7, and 10 days after extraction, animals were fixed and the TG was removed. Cryosections of the ganglia were immunostained with antibodies against glial fibrillary acidic protein (GFAP), a marker of activated SGCs, and ATF3, a marker of damaged neurons. After tooth extraction, the number of ATF3-immunoreactive (IR) neurons enclosed by GFAP-IR SGCs had increased in a time-dependent manner in the maxillary nerve region of the TG. Although ATF3-IR neurons were not detected in the mandibular nerve region, the number of GFAP-IR SGCs increased in both the maxillary and mandibular nerve regions. Our results suggest that peripheral nerve injury affects the activation of TG neurons and the SGCs around the injured neurons. Moreover, our data suggest the existence of a neuronal interaction between maxillary and mandibular neurons via SGC activation.
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spelling pubmed-27751052009-11-16 Activation of Satellite Glial Cells in Rat Trigeminal Ganglion after Upper Molar Extraction Gunjigake, Kaori K. Goto, Tetsuya Nakao, Kayoko Kobayashi, Shigeru Yamaguchi, Kazunori Acta Histochem Cytochem Regular Article The neurons in the trigeminal ganglion (TG) are surrounded by satellite glial cells (SGCs), which passively support the function of the neurons, but little is known about the interactions between SGCs and TG neurons after peripheral nerve injury. To examine the effect of nerve injury on SGCs, we investigated the activation of SGCs after neuronal damage due to the extraction of the upper molars in rats. Three, 7, and 10 days after extraction, animals were fixed and the TG was removed. Cryosections of the ganglia were immunostained with antibodies against glial fibrillary acidic protein (GFAP), a marker of activated SGCs, and ATF3, a marker of damaged neurons. After tooth extraction, the number of ATF3-immunoreactive (IR) neurons enclosed by GFAP-IR SGCs had increased in a time-dependent manner in the maxillary nerve region of the TG. Although ATF3-IR neurons were not detected in the mandibular nerve region, the number of GFAP-IR SGCs increased in both the maxillary and mandibular nerve regions. Our results suggest that peripheral nerve injury affects the activation of TG neurons and the SGCs around the injured neurons. Moreover, our data suggest the existence of a neuronal interaction between maxillary and mandibular neurons via SGC activation. Japan Society of Histochemistry and Cytochemistry 2009-10-30 2009-09-15 /pmc/articles/PMC2775105/ /pubmed/19918323 http://dx.doi.org/10.1267/ahc.09017 Text en © 2009 The Japan Society of Histochemistry and Cytochemistry This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Regular Article
Gunjigake, Kaori K.
Goto, Tetsuya
Nakao, Kayoko
Kobayashi, Shigeru
Yamaguchi, Kazunori
Activation of Satellite Glial Cells in Rat Trigeminal Ganglion after Upper Molar Extraction
title Activation of Satellite Glial Cells in Rat Trigeminal Ganglion after Upper Molar Extraction
title_full Activation of Satellite Glial Cells in Rat Trigeminal Ganglion after Upper Molar Extraction
title_fullStr Activation of Satellite Glial Cells in Rat Trigeminal Ganglion after Upper Molar Extraction
title_full_unstemmed Activation of Satellite Glial Cells in Rat Trigeminal Ganglion after Upper Molar Extraction
title_short Activation of Satellite Glial Cells in Rat Trigeminal Ganglion after Upper Molar Extraction
title_sort activation of satellite glial cells in rat trigeminal ganglion after upper molar extraction
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2775105/
https://www.ncbi.nlm.nih.gov/pubmed/19918323
http://dx.doi.org/10.1267/ahc.09017
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