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Comprehensive and Rapid Real-Time PCR Analysis of 21 Foodborne Outbreaks

A set of four duplex SYBR Green I PCR (SG-PCR) assay combined with DNA extraction using QIAamp DNA Stool Mini kit was evaluated for the detection of foodborne bacteria from 21 foodborne outbreaks. The causative pathogens were detected in almost all cases in 2 hours or less. The first run was for the...

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Detalles Bibliográficos
Autores principales: Fukushima, Hiroshi, Katsube, Kazunori, Tsunomori, Yoshie, Kishi, Ryoko, Atsuta, Junko, Akiba, Yuko
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2775201/
https://www.ncbi.nlm.nih.gov/pubmed/20016673
http://dx.doi.org/10.1155/2009/917623