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Effect of magnetic nanoparticles of Fe(3)O(4) and 5-bromotetrandrine on reversal of multidrug resistance in K562/A02 leukemic cells
This study aims to evaluate the multidrug resistance (MDR) reversal activity by magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) and 5-bromotetrandrine (BrTet) MDR cell line K562/A02 solitarily or symphysially. The proliferation of K562 and K562/A02 cells and the cytotoxicity on peripheral blood...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Dove Medical Press
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2775691/ https://www.ncbi.nlm.nih.gov/pubmed/19918367 |
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author | Cheng, Jian Wu, Weiwei Chen, Bao-an Gao, Feng Xu, Wenlin Gao, Chong Ding, Jiahua Sun, Yunyu Song, Huihui Bao, Wen Sun, Xinchen Xu, Cuirong Chen, Wenji Chen, Ningna Liu, Lijie Xia, Guohua Li, Xiaomao Wang, Xuemei |
author_facet | Cheng, Jian Wu, Weiwei Chen, Bao-an Gao, Feng Xu, Wenlin Gao, Chong Ding, Jiahua Sun, Yunyu Song, Huihui Bao, Wen Sun, Xinchen Xu, Cuirong Chen, Wenji Chen, Ningna Liu, Lijie Xia, Guohua Li, Xiaomao Wang, Xuemei |
author_sort | Cheng, Jian |
collection | PubMed |
description | This study aims to evaluate the multidrug resistance (MDR) reversal activity by magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) and 5-bromotetrandrine (BrTet) MDR cell line K562/A02 solitarily or symphysially. The proliferation of K562 and K562/A02 cells and the cytotoxicity on peripheral blood mononuclear cells (PMBCs) were evaluated by MTT assay. Cellular accumulation of daunorubicin (DNR) was analyzed by flow cytometry. Real-time polymerase chain reaction and Western blotting analyses were performed to examine the mRNA and protein levels of mdr1, respectively. The results showed that the combination of MNPs-Fe(3)O(4) and BrTet with effective concentrations significantly increased cytotoxicity against MDR cell line K562/A02. Both BrTet and MNPs-Fe(3)O(4) increased the intracellular DNR accumulation in the K562/A02 cell line, and downregulated the level of mdr1 gene and expression of P-glycoprotein. Furthermore, the combination did not have significant cytotoxicity in PMBCs. We propose that MNPs-Fe(3)O(4) conjugated with DNR and BrTet probably have synergetic effects on MDR reversal. |
format | Text |
id | pubmed-2775691 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-27756912009-11-16 Effect of magnetic nanoparticles of Fe(3)O(4) and 5-bromotetrandrine on reversal of multidrug resistance in K562/A02 leukemic cells Cheng, Jian Wu, Weiwei Chen, Bao-an Gao, Feng Xu, Wenlin Gao, Chong Ding, Jiahua Sun, Yunyu Song, Huihui Bao, Wen Sun, Xinchen Xu, Cuirong Chen, Wenji Chen, Ningna Liu, Lijie Xia, Guohua Li, Xiaomao Wang, Xuemei Int J Nanomedicine Original Research This study aims to evaluate the multidrug resistance (MDR) reversal activity by magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) and 5-bromotetrandrine (BrTet) MDR cell line K562/A02 solitarily or symphysially. The proliferation of K562 and K562/A02 cells and the cytotoxicity on peripheral blood mononuclear cells (PMBCs) were evaluated by MTT assay. Cellular accumulation of daunorubicin (DNR) was analyzed by flow cytometry. Real-time polymerase chain reaction and Western blotting analyses were performed to examine the mRNA and protein levels of mdr1, respectively. The results showed that the combination of MNPs-Fe(3)O(4) and BrTet with effective concentrations significantly increased cytotoxicity against MDR cell line K562/A02. Both BrTet and MNPs-Fe(3)O(4) increased the intracellular DNR accumulation in the K562/A02 cell line, and downregulated the level of mdr1 gene and expression of P-glycoprotein. Furthermore, the combination did not have significant cytotoxicity in PMBCs. We propose that MNPs-Fe(3)O(4) conjugated with DNR and BrTet probably have synergetic effects on MDR reversal. Dove Medical Press 2009 2009-10-19 /pmc/articles/PMC2775691/ /pubmed/19918367 Text en © 2009 Cheng et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Cheng, Jian Wu, Weiwei Chen, Bao-an Gao, Feng Xu, Wenlin Gao, Chong Ding, Jiahua Sun, Yunyu Song, Huihui Bao, Wen Sun, Xinchen Xu, Cuirong Chen, Wenji Chen, Ningna Liu, Lijie Xia, Guohua Li, Xiaomao Wang, Xuemei Effect of magnetic nanoparticles of Fe(3)O(4) and 5-bromotetrandrine on reversal of multidrug resistance in K562/A02 leukemic cells |
title | Effect of magnetic nanoparticles of Fe(3)O(4) and 5-bromotetrandrine on reversal of multidrug resistance in K562/A02 leukemic cells |
title_full | Effect of magnetic nanoparticles of Fe(3)O(4) and 5-bromotetrandrine on reversal of multidrug resistance in K562/A02 leukemic cells |
title_fullStr | Effect of magnetic nanoparticles of Fe(3)O(4) and 5-bromotetrandrine on reversal of multidrug resistance in K562/A02 leukemic cells |
title_full_unstemmed | Effect of magnetic nanoparticles of Fe(3)O(4) and 5-bromotetrandrine on reversal of multidrug resistance in K562/A02 leukemic cells |
title_short | Effect of magnetic nanoparticles of Fe(3)O(4) and 5-bromotetrandrine on reversal of multidrug resistance in K562/A02 leukemic cells |
title_sort | effect of magnetic nanoparticles of fe(3)o(4) and 5-bromotetrandrine on reversal of multidrug resistance in k562/a02 leukemic cells |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2775691/ https://www.ncbi.nlm.nih.gov/pubmed/19918367 |
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