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Water-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis
Recently, it has been demonstrated that oxygen free radicals have an important role as a signaling messenger in the receptor activator NFκB (RANK) signal pathway required for osteoclast differentiation. The aim of this study was to examine the potential of a strong free-radical scavenger, water-solu...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Dove Medical Press
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2775694/ https://www.ncbi.nlm.nih.gov/pubmed/19918370 |
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author | Yudoh, Kazuo Karasawa, Rie Masuko, Kayo Kato, Tomohiro |
author_facet | Yudoh, Kazuo Karasawa, Rie Masuko, Kayo Kato, Tomohiro |
author_sort | Yudoh, Kazuo |
collection | PubMed |
description | Recently, it has been demonstrated that oxygen free radicals have an important role as a signaling messenger in the receptor activator NFκB (RANK) signal pathway required for osteoclast differentiation. The aim of this study was to examine the potential of a strong free-radical scavenger, water-soluble fullerene (C60), as a protective agent against the RANK-induced osteoclastogenesis and osteoclastic bone destruction in arthritis, both in vitro and in vivo. The effects of C60 on the RANK-induced osteoclastogenesis and osteoclastic bone resorption were examined in vitro. Adjuvant-induced arthritic rats were used as an animal model of arthritis. Rats were divided into two subgroups: control and treatment with C60 at 1.0 μM. The left ankle joint was injected intra-articularly with water-soluble C60 (20 μl) in the C60-treated group, while, as a control, the left ankle joint in the control rats received phosphate-buffered saline (20 μl) once weekly for eight weeks. Ankle joint tissues were prepared for histologic analysis. C60 significantly inhibited the responses of osteoclast precursor cells to RANK ligand, including osteoclast differentiation and osteoclastic bone resorption in vitro. In adjuvant-induced arthritic rats, intra-articular treatment with C60 in vivo reduced the number of osteoclasts and alleviated bone resorption and destruction in the joints, while control ankle joints showed progression of joint destruction with time. These findings indicate that C60 downregulates the RANK-induced osteoclast differentiation and is a potential therapeutic agent for inhibition of osteoclastic bone destruction in arthritis. |
format | Text |
id | pubmed-2775694 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-27756942009-11-16 Water-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis Yudoh, Kazuo Karasawa, Rie Masuko, Kayo Kato, Tomohiro Int J Nanomedicine Original Research Recently, it has been demonstrated that oxygen free radicals have an important role as a signaling messenger in the receptor activator NFκB (RANK) signal pathway required for osteoclast differentiation. The aim of this study was to examine the potential of a strong free-radical scavenger, water-soluble fullerene (C60), as a protective agent against the RANK-induced osteoclastogenesis and osteoclastic bone destruction in arthritis, both in vitro and in vivo. The effects of C60 on the RANK-induced osteoclastogenesis and osteoclastic bone resorption were examined in vitro. Adjuvant-induced arthritic rats were used as an animal model of arthritis. Rats were divided into two subgroups: control and treatment with C60 at 1.0 μM. The left ankle joint was injected intra-articularly with water-soluble C60 (20 μl) in the C60-treated group, while, as a control, the left ankle joint in the control rats received phosphate-buffered saline (20 μl) once weekly for eight weeks. Ankle joint tissues were prepared for histologic analysis. C60 significantly inhibited the responses of osteoclast precursor cells to RANK ligand, including osteoclast differentiation and osteoclastic bone resorption in vitro. In adjuvant-induced arthritic rats, intra-articular treatment with C60 in vivo reduced the number of osteoclasts and alleviated bone resorption and destruction in the joints, while control ankle joints showed progression of joint destruction with time. These findings indicate that C60 downregulates the RANK-induced osteoclast differentiation and is a potential therapeutic agent for inhibition of osteoclastic bone destruction in arthritis. Dove Medical Press 2009 2009-10-19 /pmc/articles/PMC2775694/ /pubmed/19918370 Text en © 2009 Yudoh et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Yudoh, Kazuo Karasawa, Rie Masuko, Kayo Kato, Tomohiro Water-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis |
title | Water-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis |
title_full | Water-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis |
title_fullStr | Water-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis |
title_full_unstemmed | Water-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis |
title_short | Water-soluble fullerene (C60) inhibits the osteoclast differentiation and bone destruction in arthritis |
title_sort | water-soluble fullerene (c60) inhibits the osteoclast differentiation and bone destruction in arthritis |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2775694/ https://www.ncbi.nlm.nih.gov/pubmed/19918370 |
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