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Circulating levels of matrix metalloproteinase-9 (MMP-9), neutrophil gelatinase-associated lipocalin (NGAL) and their complex MMP-9/NGAL in breast cancer disease

BACKGROUND: Recent evidence suggests that neutrophil gelatinase-associated lipocalin (NGAL) expression is induced in many types of human cancer, while detection of its complex with matrix metalloproteinase-9 (MMP-9) is correlated with cancer disease status. We aim to evaluate the serum expression of...

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Autores principales: Provatopoulou, Xeni, Gounaris, Antonia, Kalogera, Eleni, Zagouri, Flora, Flessas, Ioannis, Goussetis, Evgenios, Nonni, Afroditi, Papassotiriou, Ioannis, Zografos, George
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2775750/
https://www.ncbi.nlm.nih.gov/pubmed/19889214
http://dx.doi.org/10.1186/1471-2407-9-390
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author Provatopoulou, Xeni
Gounaris, Antonia
Kalogera, Eleni
Zagouri, Flora
Flessas, Ioannis
Goussetis, Evgenios
Nonni, Afroditi
Papassotiriou, Ioannis
Zografos, George
author_facet Provatopoulou, Xeni
Gounaris, Antonia
Kalogera, Eleni
Zagouri, Flora
Flessas, Ioannis
Goussetis, Evgenios
Nonni, Afroditi
Papassotiriou, Ioannis
Zografos, George
author_sort Provatopoulou, Xeni
collection PubMed
description BACKGROUND: Recent evidence suggests that neutrophil gelatinase-associated lipocalin (NGAL) expression is induced in many types of human cancer, while detection of its complex with matrix metalloproteinase-9 (MMP-9) is correlated with cancer disease status. We aim to evaluate the serum expression of MMP-9, NGAL and their complex (MMP-9/NGAL) during the diagnostic work-up of women with breast abnormalities and investigate their correlation with disease severity. METHODS: The study included 113 women with non-palpable breast lesions undergoing vacuum-assisted breast biopsy for histological diagnosis, and 30 healthy women, which served as controls. Expression levels of MMP-9, NGAL and their complex MMP-9/NGAL were determined in peripheral blood samples with immunoenzymatic assays. RESULTS: Women with invasive ductal carcinoma exhibited significantly increased levels of MMP-9, NGAL and MMP-9/NGAL compared to healthy controls (MMP-9: p < 0.003, NGAL: p < 0.008 MMP-9/NGAL: p < 0.01). Significant correlations were observed between MMP-9 and NGAL serum levels and breast disease severity score (r = 0.229, p < 0.006 and r = 0.206, p < 0.01, respectively), whereas a non-significant correlation was found for their complex. MMP-9, NGAL and their complex MMP-9/NGAL levels were not correlated with either Body Mass Index (BMI) or age of patients. CONCLUSION: These findings suggest that the serum measurement of MMP-9 and NGAL may be useful in non-invasively monitoring breast cancer progression, while supporting their potential role as early biomarkers of breast disease status.
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spelling pubmed-27757502009-11-11 Circulating levels of matrix metalloproteinase-9 (MMP-9), neutrophil gelatinase-associated lipocalin (NGAL) and their complex MMP-9/NGAL in breast cancer disease Provatopoulou, Xeni Gounaris, Antonia Kalogera, Eleni Zagouri, Flora Flessas, Ioannis Goussetis, Evgenios Nonni, Afroditi Papassotiriou, Ioannis Zografos, George BMC Cancer Research Article BACKGROUND: Recent evidence suggests that neutrophil gelatinase-associated lipocalin (NGAL) expression is induced in many types of human cancer, while detection of its complex with matrix metalloproteinase-9 (MMP-9) is correlated with cancer disease status. We aim to evaluate the serum expression of MMP-9, NGAL and their complex (MMP-9/NGAL) during the diagnostic work-up of women with breast abnormalities and investigate their correlation with disease severity. METHODS: The study included 113 women with non-palpable breast lesions undergoing vacuum-assisted breast biopsy for histological diagnosis, and 30 healthy women, which served as controls. Expression levels of MMP-9, NGAL and their complex MMP-9/NGAL were determined in peripheral blood samples with immunoenzymatic assays. RESULTS: Women with invasive ductal carcinoma exhibited significantly increased levels of MMP-9, NGAL and MMP-9/NGAL compared to healthy controls (MMP-9: p < 0.003, NGAL: p < 0.008 MMP-9/NGAL: p < 0.01). Significant correlations were observed between MMP-9 and NGAL serum levels and breast disease severity score (r = 0.229, p < 0.006 and r = 0.206, p < 0.01, respectively), whereas a non-significant correlation was found for their complex. MMP-9, NGAL and their complex MMP-9/NGAL levels were not correlated with either Body Mass Index (BMI) or age of patients. CONCLUSION: These findings suggest that the serum measurement of MMP-9 and NGAL may be useful in non-invasively monitoring breast cancer progression, while supporting their potential role as early biomarkers of breast disease status. BioMed Central 2009-11-04 /pmc/articles/PMC2775750/ /pubmed/19889214 http://dx.doi.org/10.1186/1471-2407-9-390 Text en Copyright ©2009 Provatopoulou et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Provatopoulou, Xeni
Gounaris, Antonia
Kalogera, Eleni
Zagouri, Flora
Flessas, Ioannis
Goussetis, Evgenios
Nonni, Afroditi
Papassotiriou, Ioannis
Zografos, George
Circulating levels of matrix metalloproteinase-9 (MMP-9), neutrophil gelatinase-associated lipocalin (NGAL) and their complex MMP-9/NGAL in breast cancer disease
title Circulating levels of matrix metalloproteinase-9 (MMP-9), neutrophil gelatinase-associated lipocalin (NGAL) and their complex MMP-9/NGAL in breast cancer disease
title_full Circulating levels of matrix metalloproteinase-9 (MMP-9), neutrophil gelatinase-associated lipocalin (NGAL) and their complex MMP-9/NGAL in breast cancer disease
title_fullStr Circulating levels of matrix metalloproteinase-9 (MMP-9), neutrophil gelatinase-associated lipocalin (NGAL) and their complex MMP-9/NGAL in breast cancer disease
title_full_unstemmed Circulating levels of matrix metalloproteinase-9 (MMP-9), neutrophil gelatinase-associated lipocalin (NGAL) and their complex MMP-9/NGAL in breast cancer disease
title_short Circulating levels of matrix metalloproteinase-9 (MMP-9), neutrophil gelatinase-associated lipocalin (NGAL) and their complex MMP-9/NGAL in breast cancer disease
title_sort circulating levels of matrix metalloproteinase-9 (mmp-9), neutrophil gelatinase-associated lipocalin (ngal) and their complex mmp-9/ngal in breast cancer disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2775750/
https://www.ncbi.nlm.nih.gov/pubmed/19889214
http://dx.doi.org/10.1186/1471-2407-9-390
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