A Highly Sensitive, Quick and Simple Quantification Method for Nicotianamine and 2′-Deoxymugineic Acid from Minimum Samples Using LC/ESI-TOF-MS Achieves Functional Analysis of These Components in Plants

A highly sensitive quantitative method for assaying nicotianamine (NA) and 2′-deoxymugineic acid (DMA) using liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) was developed. The amino and hydroxyl groups of NA and DMA were derivatized using 9-fluorenylmet...

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Autores principales: Kakei, Yusuke, Yamaguchi, Isomaro, Kobayashi, Takanori, Takahashi, Michiko, Nakanishi, Hiromi, Yamakawa, Takashi, Nishizawa, Naoko K.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2775962/
https://www.ncbi.nlm.nih.gov/pubmed/19880395
http://dx.doi.org/10.1093/pcp/pcp141
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author Kakei, Yusuke
Yamaguchi, Isomaro
Kobayashi, Takanori
Takahashi, Michiko
Nakanishi, Hiromi
Yamakawa, Takashi
Nishizawa, Naoko K.
author_facet Kakei, Yusuke
Yamaguchi, Isomaro
Kobayashi, Takanori
Takahashi, Michiko
Nakanishi, Hiromi
Yamakawa, Takashi
Nishizawa, Naoko K.
author_sort Kakei, Yusuke
collection PubMed
description A highly sensitive quantitative method for assaying nicotianamine (NA) and 2′-deoxymugineic acid (DMA) using liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) was developed. The amino and hydroxyl groups of NA and DMA were derivatized using 9-fluorenylmethoxycarbonyl chloride. The amounts of NA and DMA in 10 μl of xylem sap from rice cultivated under iron (Fe)-sufficient and Fe-deficient conditions were quantified without concentration. In Fe-sufficient plants, the concentrations of NA and DMA were almost equal to that of Fe. In Fe-deficient plants, the concentration of NA did not change significantly, whereas that of DMA increased markedly.
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spelling pubmed-27759622009-11-12 A Highly Sensitive, Quick and Simple Quantification Method for Nicotianamine and 2′-Deoxymugineic Acid from Minimum Samples Using LC/ESI-TOF-MS Achieves Functional Analysis of These Components in Plants Kakei, Yusuke Yamaguchi, Isomaro Kobayashi, Takanori Takahashi, Michiko Nakanishi, Hiromi Yamakawa, Takashi Nishizawa, Naoko K. Plant Cell Physiol Short Communications A highly sensitive quantitative method for assaying nicotianamine (NA) and 2′-deoxymugineic acid (DMA) using liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) was developed. The amino and hydroxyl groups of NA and DMA were derivatized using 9-fluorenylmethoxycarbonyl chloride. The amounts of NA and DMA in 10 μl of xylem sap from rice cultivated under iron (Fe)-sufficient and Fe-deficient conditions were quantified without concentration. In Fe-sufficient plants, the concentrations of NA and DMA were almost equal to that of Fe. In Fe-deficient plants, the concentration of NA did not change significantly, whereas that of DMA increased markedly. Oxford University Press 2009-11 2009-10-30 /pmc/articles/PMC2775962/ /pubmed/19880395 http://dx.doi.org/10.1093/pcp/pcp141 Text en © The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. http://creativecommons.org/licenses/by-nc/2.5/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communications
Kakei, Yusuke
Yamaguchi, Isomaro
Kobayashi, Takanori
Takahashi, Michiko
Nakanishi, Hiromi
Yamakawa, Takashi
Nishizawa, Naoko K.
A Highly Sensitive, Quick and Simple Quantification Method for Nicotianamine and 2′-Deoxymugineic Acid from Minimum Samples Using LC/ESI-TOF-MS Achieves Functional Analysis of These Components in Plants
title A Highly Sensitive, Quick and Simple Quantification Method for Nicotianamine and 2′-Deoxymugineic Acid from Minimum Samples Using LC/ESI-TOF-MS Achieves Functional Analysis of These Components in Plants
title_full A Highly Sensitive, Quick and Simple Quantification Method for Nicotianamine and 2′-Deoxymugineic Acid from Minimum Samples Using LC/ESI-TOF-MS Achieves Functional Analysis of These Components in Plants
title_fullStr A Highly Sensitive, Quick and Simple Quantification Method for Nicotianamine and 2′-Deoxymugineic Acid from Minimum Samples Using LC/ESI-TOF-MS Achieves Functional Analysis of These Components in Plants
title_full_unstemmed A Highly Sensitive, Quick and Simple Quantification Method for Nicotianamine and 2′-Deoxymugineic Acid from Minimum Samples Using LC/ESI-TOF-MS Achieves Functional Analysis of These Components in Plants
title_short A Highly Sensitive, Quick and Simple Quantification Method for Nicotianamine and 2′-Deoxymugineic Acid from Minimum Samples Using LC/ESI-TOF-MS Achieves Functional Analysis of These Components in Plants
title_sort highly sensitive, quick and simple quantification method for nicotianamine and 2′-deoxymugineic acid from minimum samples using lc/esi-tof-ms achieves functional analysis of these components in plants
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2775962/
https://www.ncbi.nlm.nih.gov/pubmed/19880395
http://dx.doi.org/10.1093/pcp/pcp141
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