Cargando…

Basonuclin-2 Requirements for Zebrafish Adult Pigment Pattern Development and Female Fertility

Relatively little is known about the generation of adult form. One complex adult trait that is particularly amenable to genetic and experimental analysis is the zebrafish pigment pattern, which undergoes extensive remodeling during post-embryonic development to form adult stripes. These stripes resu...

Descripción completa

Detalles Bibliográficos
Autores principales: Lang, Michael R., Patterson, Larissa B., Gordon, Tiffany N., Johnson, Stephen L., Parichy, David M.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2776513/
https://www.ncbi.nlm.nih.gov/pubmed/19956727
http://dx.doi.org/10.1371/journal.pgen.1000744
_version_ 1782174102756261888
author Lang, Michael R.
Patterson, Larissa B.
Gordon, Tiffany N.
Johnson, Stephen L.
Parichy, David M.
author_facet Lang, Michael R.
Patterson, Larissa B.
Gordon, Tiffany N.
Johnson, Stephen L.
Parichy, David M.
author_sort Lang, Michael R.
collection PubMed
description Relatively little is known about the generation of adult form. One complex adult trait that is particularly amenable to genetic and experimental analysis is the zebrafish pigment pattern, which undergoes extensive remodeling during post-embryonic development to form adult stripes. These stripes result from the arrangement of three classes of neural crest-derived pigment cells, or chromatophores: melanophores, xanthophores, and iridophores. Here, we analyze the zebrafish bonaparte mutant, which has a normal early pigment pattern but exhibits a severe disruption to the adult stripe pattern. We show that the bonaparte mutant phenotype arises from mutations in basonuclin-2 (bnc2), encoding a highly conserved, nuclear-localized zinc finger protein of unknown function. We show that bnc2 acts non-autonomously to the melanophore lineage and is expressed by hypodermal cells adjacent to chromatophores during adult pigment pattern formation. In bonaparte (bnc2) mutants, all three types of chromatophores differentiate but then are lost by extrusion through the skin. We further show that while bnc2 promotes the development of two genetically distinct populations of melanophores in the body stripes, chromatophores of the fins and scales remain unaffected in bonaparte mutants, though a requirement of fin chromatophores for bnc2 is revealed in the absence of kit and colony stimulating factor-1 receptor activity. Finally, we find that bonaparte (bnc2) mutants exhibit dysmorphic ovaries correlating with infertility and bnc2 is expressed in somatic ovarian cells, whereas the related gene, bnc1, is expressed within oocytes; and we find that both bnc2 and bnc1 are expressed abundantly within the central nervous system. These findings identify bnc2 as an important mediator of adult pigment pattern formation and identify bonaparte mutants as an animal model for dissecting bnc2 functions.
format Text
id pubmed-2776513
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-27765132009-12-03 Basonuclin-2 Requirements for Zebrafish Adult Pigment Pattern Development and Female Fertility Lang, Michael R. Patterson, Larissa B. Gordon, Tiffany N. Johnson, Stephen L. Parichy, David M. PLoS Genet Research Article Relatively little is known about the generation of adult form. One complex adult trait that is particularly amenable to genetic and experimental analysis is the zebrafish pigment pattern, which undergoes extensive remodeling during post-embryonic development to form adult stripes. These stripes result from the arrangement of three classes of neural crest-derived pigment cells, or chromatophores: melanophores, xanthophores, and iridophores. Here, we analyze the zebrafish bonaparte mutant, which has a normal early pigment pattern but exhibits a severe disruption to the adult stripe pattern. We show that the bonaparte mutant phenotype arises from mutations in basonuclin-2 (bnc2), encoding a highly conserved, nuclear-localized zinc finger protein of unknown function. We show that bnc2 acts non-autonomously to the melanophore lineage and is expressed by hypodermal cells adjacent to chromatophores during adult pigment pattern formation. In bonaparte (bnc2) mutants, all three types of chromatophores differentiate but then are lost by extrusion through the skin. We further show that while bnc2 promotes the development of two genetically distinct populations of melanophores in the body stripes, chromatophores of the fins and scales remain unaffected in bonaparte mutants, though a requirement of fin chromatophores for bnc2 is revealed in the absence of kit and colony stimulating factor-1 receptor activity. Finally, we find that bonaparte (bnc2) mutants exhibit dysmorphic ovaries correlating with infertility and bnc2 is expressed in somatic ovarian cells, whereas the related gene, bnc1, is expressed within oocytes; and we find that both bnc2 and bnc1 are expressed abundantly within the central nervous system. These findings identify bnc2 as an important mediator of adult pigment pattern formation and identify bonaparte mutants as an animal model for dissecting bnc2 functions. Public Library of Science 2009-11-26 /pmc/articles/PMC2776513/ /pubmed/19956727 http://dx.doi.org/10.1371/journal.pgen.1000744 Text en Lang et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lang, Michael R.
Patterson, Larissa B.
Gordon, Tiffany N.
Johnson, Stephen L.
Parichy, David M.
Basonuclin-2 Requirements for Zebrafish Adult Pigment Pattern Development and Female Fertility
title Basonuclin-2 Requirements for Zebrafish Adult Pigment Pattern Development and Female Fertility
title_full Basonuclin-2 Requirements for Zebrafish Adult Pigment Pattern Development and Female Fertility
title_fullStr Basonuclin-2 Requirements for Zebrafish Adult Pigment Pattern Development and Female Fertility
title_full_unstemmed Basonuclin-2 Requirements for Zebrafish Adult Pigment Pattern Development and Female Fertility
title_short Basonuclin-2 Requirements for Zebrafish Adult Pigment Pattern Development and Female Fertility
title_sort basonuclin-2 requirements for zebrafish adult pigment pattern development and female fertility
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2776513/
https://www.ncbi.nlm.nih.gov/pubmed/19956727
http://dx.doi.org/10.1371/journal.pgen.1000744
work_keys_str_mv AT langmichaelr basonuclin2requirementsforzebrafishadultpigmentpatterndevelopmentandfemalefertility
AT pattersonlarissab basonuclin2requirementsforzebrafishadultpigmentpatterndevelopmentandfemalefertility
AT gordontiffanyn basonuclin2requirementsforzebrafishadultpigmentpatterndevelopmentandfemalefertility
AT johnsonstephenl basonuclin2requirementsforzebrafishadultpigmentpatterndevelopmentandfemalefertility
AT parichydavidm basonuclin2requirementsforzebrafishadultpigmentpatterndevelopmentandfemalefertility