NMR assignment of the nonstructural protein nsp3(1066–1181) from SARS-CoV

Sequence-specific NMR assignments of the globular core comprising the residues 1066–1181 within the non-structural protein nsp3e from the SARS coronavirus have been obtained using triple-resonance NMR experiments with the uniformly [(13)C, (15)N]-labeled protein. The backbone and side chain assignme...

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Detalles Bibliográficos
Autores principales: Serrano, Pedro, Johnson, Margaret A., Chatterjee, Amarnath, Pedrini, Bill, Wüthrich, Kurt
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2776825/
https://www.ncbi.nlm.nih.gov/pubmed/19636888
http://dx.doi.org/10.1007/s12104-008-9104-x
Descripción
Sumario:Sequence-specific NMR assignments of the globular core comprising the residues 1066–1181 within the non-structural protein nsp3e from the SARS coronavirus have been obtained using triple-resonance NMR experiments with the uniformly [(13)C, (15)N]-labeled protein. The backbone and side chain assignments are nearly complete, providing the basis for the ongoing NMR structure determination. A preliminary identification of regular secondary structures has been derived from the (13)C chemical shifts.

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