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Gradient lithography of engineered proteins to fabricate 2D and 3D cell culture microenvironments

Spatial patterning of proteins is a valuable technique for many biological applications and is the prevailing tool for defining microenvironments for cells in culture, a required procedure in developmental biology and tissue engineering research. However, it is still challenging to achieve protein p...

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Detalles Bibliográficos
Autores principales: Wang, Sheng, Wong Po Foo, Cheryl, Warrier, Ajithkumar, Poo, Mu-ming, Heilshorn, Sarah C., Zhang, Xiang
Formato: Texto
Lenguaje:English
Publicado: Springer US 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777213/
https://www.ncbi.nlm.nih.gov/pubmed/19495986
http://dx.doi.org/10.1007/s10544-009-9329-1
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author Wang, Sheng
Wong Po Foo, Cheryl
Warrier, Ajithkumar
Poo, Mu-ming
Heilshorn, Sarah C.
Zhang, Xiang
author_facet Wang, Sheng
Wong Po Foo, Cheryl
Warrier, Ajithkumar
Poo, Mu-ming
Heilshorn, Sarah C.
Zhang, Xiang
author_sort Wang, Sheng
collection PubMed
description Spatial patterning of proteins is a valuable technique for many biological applications and is the prevailing tool for defining microenvironments for cells in culture, a required procedure in developmental biology and tissue engineering research. However, it is still challenging to achieve protein patterns that closely mimic native microenvironments, such as gradient protein distributions with desirable mechanical properties. By combining projection dynamic mask lithography and protein engineering with non-canonical photosensitive amino acids, we demonstrate a simple, scalable strategy to fabricate any user-defined 2D or 3D stable gradient pattern with complex geometries from an artificial extracellular matrix (aECM) protein. We show that the elastic modulus and chemical nature of the gradient profile are biocompatible and allow useful applications in cell biological research.
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spelling pubmed-27772132009-11-17 Gradient lithography of engineered proteins to fabricate 2D and 3D cell culture microenvironments Wang, Sheng Wong Po Foo, Cheryl Warrier, Ajithkumar Poo, Mu-ming Heilshorn, Sarah C. Zhang, Xiang Biomed Microdevices Article Spatial patterning of proteins is a valuable technique for many biological applications and is the prevailing tool for defining microenvironments for cells in culture, a required procedure in developmental biology and tissue engineering research. However, it is still challenging to achieve protein patterns that closely mimic native microenvironments, such as gradient protein distributions with desirable mechanical properties. By combining projection dynamic mask lithography and protein engineering with non-canonical photosensitive amino acids, we demonstrate a simple, scalable strategy to fabricate any user-defined 2D or 3D stable gradient pattern with complex geometries from an artificial extracellular matrix (aECM) protein. We show that the elastic modulus and chemical nature of the gradient profile are biocompatible and allow useful applications in cell biological research. Springer US 2009-06-03 2009 /pmc/articles/PMC2777213/ /pubmed/19495986 http://dx.doi.org/10.1007/s10544-009-9329-1 Text en © The Author(s) 2009 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Wang, Sheng
Wong Po Foo, Cheryl
Warrier, Ajithkumar
Poo, Mu-ming
Heilshorn, Sarah C.
Zhang, Xiang
Gradient lithography of engineered proteins to fabricate 2D and 3D cell culture microenvironments
title Gradient lithography of engineered proteins to fabricate 2D and 3D cell culture microenvironments
title_full Gradient lithography of engineered proteins to fabricate 2D and 3D cell culture microenvironments
title_fullStr Gradient lithography of engineered proteins to fabricate 2D and 3D cell culture microenvironments
title_full_unstemmed Gradient lithography of engineered proteins to fabricate 2D and 3D cell culture microenvironments
title_short Gradient lithography of engineered proteins to fabricate 2D and 3D cell culture microenvironments
title_sort gradient lithography of engineered proteins to fabricate 2d and 3d cell culture microenvironments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777213/
https://www.ncbi.nlm.nih.gov/pubmed/19495986
http://dx.doi.org/10.1007/s10544-009-9329-1
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