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An Initial Step of GAS-Containing Autophagosome-Like Vacuoles Formation Requires Rab7
Group A streptococcus (GAS; Streptococcus pyogenes) is a common pathogen that invades non-phagocytic human cells via endocytosis. Once taken up by cells, it escapes from the endocytic pathway to the cytoplasm, but here it is contained within a membrane-bound structure termed GAS-containing autophago...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777386/ https://www.ncbi.nlm.nih.gov/pubmed/19956673 http://dx.doi.org/10.1371/journal.ppat.1000670 |
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author | Yamaguchi, Hitomi Nakagawa, Ichiro Yamamoto, Akitsugu Amano, Atsuo Noda, Takeshi Yoshimori, Tamotsu |
author_facet | Yamaguchi, Hitomi Nakagawa, Ichiro Yamamoto, Akitsugu Amano, Atsuo Noda, Takeshi Yoshimori, Tamotsu |
author_sort | Yamaguchi, Hitomi |
collection | PubMed |
description | Group A streptococcus (GAS; Streptococcus pyogenes) is a common pathogen that invades non-phagocytic human cells via endocytosis. Once taken up by cells, it escapes from the endocytic pathway to the cytoplasm, but here it is contained within a membrane-bound structure termed GAS-containing autophagosome-like vacuoles (GcAVs). The autophagosome marker GFP-LC3 associates with GcAVs, and other components of the autophagosomal pathway are involved in GcAV formation. However, the mechanistic relationship between GcAV and canonical autophagy is largely unknown. Here, we morphologically analyzed GcAV formation in detail. Initially, a small, GFP-LC3-positive GcAV sequesters each streptococcal chain, and these then coalesce into a single, large GcAV. Expression of a dominant-negative form of Rab7 or RNAi-mediated knockdown of Rab7 prevented the initial formation of small GcAV structures. Our results demonstrate that mechanisms of GcAV formation includes not only the common machinery of autophagy, but also Rab7 as an additional component, which is dispensable in canonical autophagosome formation. |
format | Text |
id | pubmed-2777386 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-27773862009-12-03 An Initial Step of GAS-Containing Autophagosome-Like Vacuoles Formation Requires Rab7 Yamaguchi, Hitomi Nakagawa, Ichiro Yamamoto, Akitsugu Amano, Atsuo Noda, Takeshi Yoshimori, Tamotsu PLoS Pathog Research Article Group A streptococcus (GAS; Streptococcus pyogenes) is a common pathogen that invades non-phagocytic human cells via endocytosis. Once taken up by cells, it escapes from the endocytic pathway to the cytoplasm, but here it is contained within a membrane-bound structure termed GAS-containing autophagosome-like vacuoles (GcAVs). The autophagosome marker GFP-LC3 associates with GcAVs, and other components of the autophagosomal pathway are involved in GcAV formation. However, the mechanistic relationship between GcAV and canonical autophagy is largely unknown. Here, we morphologically analyzed GcAV formation in detail. Initially, a small, GFP-LC3-positive GcAV sequesters each streptococcal chain, and these then coalesce into a single, large GcAV. Expression of a dominant-negative form of Rab7 or RNAi-mediated knockdown of Rab7 prevented the initial formation of small GcAV structures. Our results demonstrate that mechanisms of GcAV formation includes not only the common machinery of autophagy, but also Rab7 as an additional component, which is dispensable in canonical autophagosome formation. Public Library of Science 2009-11-26 /pmc/articles/PMC2777386/ /pubmed/19956673 http://dx.doi.org/10.1371/journal.ppat.1000670 Text en Yamaguchi et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Yamaguchi, Hitomi Nakagawa, Ichiro Yamamoto, Akitsugu Amano, Atsuo Noda, Takeshi Yoshimori, Tamotsu An Initial Step of GAS-Containing Autophagosome-Like Vacuoles Formation Requires Rab7 |
title | An Initial Step of GAS-Containing Autophagosome-Like Vacuoles Formation Requires Rab7 |
title_full | An Initial Step of GAS-Containing Autophagosome-Like Vacuoles Formation Requires Rab7 |
title_fullStr | An Initial Step of GAS-Containing Autophagosome-Like Vacuoles Formation Requires Rab7 |
title_full_unstemmed | An Initial Step of GAS-Containing Autophagosome-Like Vacuoles Formation Requires Rab7 |
title_short | An Initial Step of GAS-Containing Autophagosome-Like Vacuoles Formation Requires Rab7 |
title_sort | initial step of gas-containing autophagosome-like vacuoles formation requires rab7 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777386/ https://www.ncbi.nlm.nih.gov/pubmed/19956673 http://dx.doi.org/10.1371/journal.ppat.1000670 |
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