High-resolution profiling of homing endonuclease binding and catalytic specificity using yeast surface display

Experimental analysis and manipulation of protein–DNA interactions pose unique biophysical challenges arising from the structural and chemical homogeneity of DNA polymers. We report the use of yeast surface display for analytical and selection-based applications for the interaction between a LAGLIDA...

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Detalles Bibliográficos
Autores principales: Jarjour, Jordan, West-Foyle, Hoku, Certo, Michael T., Hubert, Christopher G., Doyle, Lindsey, Getz, Melissa M., Stoddard, Barry L., Scharenberg, Andrew M.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777416/
https://www.ncbi.nlm.nih.gov/pubmed/19740766
http://dx.doi.org/10.1093/nar/gkp726

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